Detailed information of TA system TA064857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 7044..7566
• Replicon: plasmid pSal2
• Accession: NZ_CP016611
• Organism: Xylella fastidiosa strain Salento-2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BC375_RS12025
• Protein ID: WP_004085905.1
• Coordinates: 7279..7566 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A060HDF9
• Locus tag: BC375_RS12020
• Protein ID: WP_004085758.1
• Coordinates: 7044..7286 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC375_RS12475 (BC375_11270)	2224..2538	+	315	WP_155561293.1	EexN family lipoprotein	-
BC375_RS11985 (BC375_11275)	2544..3941	+	1398	WP_046417516.1	P-type conjugative transfer protein TrbL	-
BC375_RS11990	3947..4681	+	735	WP_232755968.1	conjugal transfer protein TrbN	-
BC375_RS11995 (BC375_11280)	4577..5056	-	480	Protein_6	Tn3 family transposase	-
BC375_RS12000 (BC375_11285)	5046..5300	+	255	WP_230950296.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BC375_RS12480	5302..5475	+	174	WP_155641416.1	hypothetical protein	-
BC375_RS12005 (BC375_11290)	5850..6176	-	327	WP_004091396.1	DNA-binding protein	-
BC375_RS12010 (BC375_11295)	6306..6626	-	321	WP_046417511.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BC375_RS12015	6763..6849	-	87	Protein_11	type II toxin-antitoxin system RelE/ParE family toxin	-
BC375_RS12020 (BC375_11300)	7044..7286	+	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BC375_RS12025 (BC375_11305)	7279..7566	+	288	WP_004085905.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BC375_RS12030 (BC375_11310)	7654..8211	+	558	WP_046417502.1	recombinase family protein	-
BC375_RS12035	8398..12513	-	4116	WP_046417500.1	zincin-like metallopeptidase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..35270	35270
	-	inside	IScluster/Tn	-	-	4559..8211	3652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11159.64 Da
• Isoelectric Point: 5.9570

>T64857 WP_004085905.1 NZ_CP016611:7279-7566 [Xylella fastidiosa]
MAKSYRLTPLAEADLEEIWFYTFRHWSIEQADSYHRSLVAVFEGLAAGTKQGCPSVLPDFNKYLCGSHVVYFMDYADHLD
IIRILHQRQDTERYL

Nucleotide

• Length: 288 bp

>T64857 NZ_CP016611:7279-7566 [Xylella fastidiosa]
ATGGCTAAATCCTATCGACTGACACCGCTGGCCGAGGCCGATCTTGAAGAAATCTGGTTCTACACATTCAGGCACTGGTC
GATAGAGCAGGCCGACAGCTACCACCGCAGCCTAGTTGCAGTTTTCGAGGGGCTGGCAGCAGGTACAAAGCAAGGCTGCC
CTTCTGTTTTGCCGGACTTCAATAAATACCTATGTGGTTCGCATGTAGTGTATTTCATGGATTACGCAGATCACTTGGAT
ATTATCCGCATCCTGCATCAGCGGCAGGATACCGAGCGGTACCTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8774.77 Da
• Isoelectric Point: 6.9881

>AT64857 WP_004085758.1 NZ_CP016611:7044-7286 [Xylella fastidiosa]
MSRNTSVALGPHFTSFIDAQVQGGRYGTASDVVRAGLRLLEEHETKVKALQDALNVGHQSGEPRPFDSEVFLSRMHTQHG

Nucleotide

• Length: 243 bp

>AT64857 NZ_CP016611:7044-7286 [Xylella fastidiosa]
ATGAGTCGAAACACATCCGTTGCCCTCGGGCCGCACTTCACCAGCTTTATTGATGCCCAAGTCCAGGGCGGTCGCTACGG
TACCGCCAGCGATGTTGTCAGGGCAGGTCTGCGGCTGCTGGAGGAACACGAAACAAAGGTGAAGGCACTGCAAGATGCGC
TGAATGTTGGCCACCAATCCGGCGAGCCGCGCCCGTTCGACAGCGAGGTGTTTTTGAGCCGGATGCACACGCAGCATGGC
TAA

Structures

Antitoxin

• AlphaFold DB: A0A060HDF9