Detailed information of TA system TA064856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2484518..2485117
• Replicon: chromosome
• Accession: NZ_CP016610
• Organism: Xylella fastidiosa strain Salento-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BC375_RS11880
• Protein ID: WP_230949896.1
• Coordinates: 2484830..2485117 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9P9V8
• Locus tag: BC375_RS11875
• Protein ID: WP_010895177.1
• Coordinates: 2484518..2484826 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC375_RS13105	2480533..2480811	+	279	WP_230949894.1	calcium-binding protein	-
BC375_RS11845 (BC375_11075)	2481446..2482207	-	762	WP_046420943.1	Bax inhibitor-1/YccA family protein	-
BC375_RS13270	2483008..2483139	+	132	WP_262363498.1	hypothetical protein	-
BC375_RS11870 (BC375_11100)	2483520..2484452	+	933	WP_230949895.1	hypothetical protein	-
BC375_RS11875 (BC375_11105)	2484518..2484826	-	309	WP_010895177.1	putative addiction module antidote protein	Antitoxin
BC375_RS11880 (BC375_11110)	2484830..2485117	-	288	WP_230949896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BC375_RS11885 (BC375_11115)	2485213..2485983	+	771	WP_046420949.1	hypothetical protein	-
BC375_RS12460 (BC375_11120)	2485983..2486252	+	270	WP_046420952.1	hypothetical protein	-
BC375_RS11895 (BC375_11125)	2486571..2486947	-	377	Protein_2224	DUF596 domain-containing protein	-
BC375_RS11900 (BC375_11130)	2486952..2487305	-	354	WP_230949871.1	DUF769 domain-containing protein	-
BC375_RS11905 (BC375_11135)	2487512..2487904	-	393	WP_046420955.1	DUF596 domain-containing protein	-
BC375_RS11910 (BC375_11140)	2487914..2489017	-	1104	WP_046420959.1	DUF769 domain-containing protein	-
BC375_RS11920 (BC375_11150)	2489454..2489831	-	378	WP_046420980.1	DUF596 domain-containing protein	-
BC375_RS11925	2489843..2490103	-	261	Protein_2229	hemagglutinin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2483520..2486252	2732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (15-100)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10940.67 Da
• Isoelectric Point: 10.3109

>T64856 WP_230949896.1 NZ_CP016610:c2485117-2484830 [Xylella fastidiosa]
VKRLEEFSDWLKGLKDGLARQRLIKRLRKVQLGNFGDVQPVGGCVFEMREHFGPGWRMYYVQRGNFLIVMLGGGDKSTQQ
SDIRRAIELAKSLED

Nucleotide

• Length: 288 bp

>T64856 NZ_CP016610:c2485117-2484830 [Xylella fastidiosa]
GTGAAACGACTCGAAGAGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCTCATCAAGCGCTT
ACGCAAGGTGCAGCTCGGGAACTTTGGAGACGTGCAGCCGGTGGGTGGATGCGTGTTTGAGATGCGCGAACACTTCGGAC
CTGGCTGGCGTATGTACTACGTGCAGCGAGGCAACTTTCTCATCGTGATGCTCGGTGGCGGCGACAAGTCAACGCAACAA
TCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTGGAGGATTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11038.60 Da
• Isoelectric Point: 4.6561

>AT64856 WP_010895177.1 NZ_CP016610:c2484826-2484518 [Xylella fastidiosa]
VTITKKINVSELPEFDAAEYLSSEEEVAAYLTAVLEENDPALLAAALGDIARSRGMSQIAKDSGITREGLYKALRPGSEP
RFDTISRVCTALGIRLVAQPMR

Nucleotide

• Length: 309 bp

>AT64856 NZ_CP016610:c2484826-2484518 [Xylella fastidiosa]
GTGACCATCACCAAGAAAATCAATGTCTCTGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAGCAGCGAGGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGCATGTCTCAGATTGCGAAGGATTCCGGCATCACGCGGGAAGGTCTATACAAGGCCCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTCGGTATACGTCTAGTAGCGCAACCAATGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S0V409