Detailed information of TA system TA064853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1296743..1297335
• Replicon: chromosome
• Accession: NZ_CP016610
• Organism: Xylella fastidiosa strain Salento-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: BC375_RS06350
• Protein ID: WP_004085590.1
• Coordinates: 1296743..1297039 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: BC375_RS06355
• Protein ID: WP_004085592.1
• Coordinates: 1297042..1297335 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC375_RS06325 (BC375_05895)	1292968..1293543	+	576	WP_232755941.1	DUF4065 domain-containing protein	-
BC375_RS06330 (BC375_05900)	1293546..1293770	+	225	WP_230949856.1	hypothetical protein	-
BC375_RS06335 (BC375_05905)	1293771..1294934	-	1164	WP_046419971.1	tail fiber protein	-
BC375_RS06340 (BC375_05910)	1294938..1295498	-	561	WP_046419969.1	DUF2612 domain-containing protein	-
BC375_RS06345 (BC375_05915)	1295495..1296640	-	1146	WP_046419967.1	baseplate J/gp47 family protein	-
BC375_RS06350 (BC375_05920)	1296743..1297039	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BC375_RS06355 (BC375_05925)	1297042..1297335	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
BC375_RS06360 (BC375_05930)	1297391..1297744	-	354	WP_046419963.1	hypothetical protein	-
BC375_RS06365 (BC375_05935)	1297741..1298382	-	642	WP_046419962.1	Gp138 family membrane-puncturing spike protein	-
BC375_RS06370 (BC375_05940)	1298379..1299209	-	831	WP_046419672.1	hypothetical protein	-
BC375_RS06375 (BC375_05945)	1299206..1299523	-	318	WP_046419669.1	hypothetical protein	-
BC375_RS12825	1299523..1299765	-	243	WP_234048933.1	hypothetical protein	-
BC375_RS06380 (BC375_05950)	1299759..1300292	-	534	WP_234048934.1	hypothetical protein	-
BC375_RS06385 (BC375_05955)	1300700..1300957	-	258	WP_046419904.1	Blp family class II bacteriocin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1290123..1337823	47700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T64853 WP_004085590.1 NZ_CP016610:1296743-1297039 [Xylella fastidiosa]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T64853 NZ_CP016610:1296743-1297039 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCGGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT64853 WP_004085592.1 NZ_CP016610:1297042-1297335 [Xylella fastidiosa]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT64853 NZ_CP016610:1297042-1297335 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAAACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCAACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTAGGGATCGAGCTGTCTGCGAAACCGTCGGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A060HCK8