Detailed information of TA system TA064847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2484319..2484918
• Replicon: chromosome
• Accession: NZ_CP016608
• Organism: Xylella fastidiosa strain Salento-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BCV75_RS11860
• Protein ID: WP_230949896.1
• Coordinates: 2484631..2484918 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9P9V8
• Locus tag: BCV75_RS11855
• Protein ID: WP_010895177.1
• Coordinates: 2484319..2484627 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV75_RS13115	2480334..2480612	+	279	WP_230949894.1	calcium-binding protein	-
BCV75_RS11825 (BCV75_11015)	2481247..2482008	-	762	WP_046420943.1	Bax inhibitor-1/YccA family protein	-
BCV75_RS13275	2482809..2482940	+	132	WP_262363498.1	hypothetical protein	-
BCV75_RS11850 (BCV75_11040)	2483321..2484253	+	933	WP_230949895.1	hypothetical protein	-
BCV75_RS11855 (BCV75_11045)	2484319..2484627	-	309	WP_010895177.1	putative addiction module antidote protein	Antitoxin
BCV75_RS11860 (BCV75_11050)	2484631..2484918	-	288	WP_230949896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCV75_RS11865 (BCV75_11055)	2485014..2485784	+	771	WP_046420949.1	hypothetical protein	-
BCV75_RS12450 (BCV75_11060)	2485784..2486053	+	270	WP_046420952.1	hypothetical protein	-
BCV75_RS11875 (BCV75_11065)	2486372..2486748	-	377	Protein_2225	DUF596 domain-containing protein	-
BCV75_RS11880 (BCV75_11070)	2486753..2487106	-	354	WP_230949871.1	DUF769 domain-containing protein	-
BCV75_RS11885 (BCV75_11075)	2487313..2487705	-	393	WP_046420955.1	DUF596 domain-containing protein	-
BCV75_RS11890 (BCV75_11080)	2487715..2488818	-	1104	WP_046420959.1	DUF769 domain-containing protein	-
BCV75_RS11895 (BCV75_11085)	2489255..2489632	-	378	WP_046420980.1	DUF596 domain-containing protein	-
BCV75_RS11900 (BCV75_11090)	2489644..2489904	-	261	Protein_2230	hemagglutinin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2483321..2486053	2732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (15-100)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10940.67 Da
• Isoelectric Point: 10.3109

>T64847 WP_230949896.1 NZ_CP016608:c2484918-2484631 [Xylella fastidiosa]
VKRLEEFSDWLKGLKDGLARQRLIKRLRKVQLGNFGDVQPVGGCVFEMREHFGPGWRMYYVQRGNFLIVMLGGGDKSTQQ
SDIRRAIELAKSLED

Nucleotide

• Length: 288 bp

>T64847 NZ_CP016608:c2484918-2484631 [Xylella fastidiosa]
GTGAAACGACTCGAAGAGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCTCATCAAGCGCTT
ACGCAAGGTGCAGCTCGGGAACTTTGGAGACGTGCAGCCGGTGGGTGGATGCGTGTTTGAGATGCGCGAACACTTCGGAC
CTGGCTGGCGTATGTACTACGTGCAGCGAGGCAACTTTCTCATCGTGATGCTCGGTGGCGGCGACAAGTCAACGCAACAA
TCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTGGAGGATTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11038.60 Da
• Isoelectric Point: 4.6561

>AT64847 WP_010895177.1 NZ_CP016608:c2484627-2484319 [Xylella fastidiosa]
VTITKKINVSELPEFDAAEYLSSEEEVAAYLTAVLEENDPALLAAALGDIARSRGMSQIAKDSGITREGLYKALRPGSEP
RFDTISRVCTALGIRLVAQPMR

Nucleotide

• Length: 309 bp

>AT64847 NZ_CP016608:c2484627-2484319 [Xylella fastidiosa]
GTGACCATCACCAAGAAAATCAATGTCTCTGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAGCAGCGAGGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGCATGTCTCAGATTGCGAAGGATTCCGGCATCACGCGGGAAGGTCTATACAAGGCCCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTCGGTATACGTCTAGTAGCGCAACCAATGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S0V409