Detailed information of TA system TA064844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1296619..1297211
• Replicon: chromosome
• Accession: NZ_CP016608
• Organism: Xylella fastidiosa strain Salento-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: BCV75_RS06330
• Protein ID: WP_004085590.1
• Coordinates: 1296619..1296915 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: BCV75_RS06335
• Protein ID: WP_004085592.1
• Coordinates: 1296918..1297211 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV75_RS06305 (BCV75_05830)	1292850..1293419	+	570	WP_046420067.1	DUF4065 domain-containing protein	-
BCV75_RS06310 (BCV75_05835)	1293422..1293646	+	225	WP_230949856.1	hypothetical protein	-
BCV75_RS06315 (BCV75_05840)	1293647..1294810	-	1164	WP_046419971.1	tail fiber protein	-
BCV75_RS06320 (BCV75_05845)	1294814..1295374	-	561	WP_046419969.1	DUF2612 domain-containing protein	-
BCV75_RS06325 (BCV75_05850)	1295371..1296516	-	1146	WP_046419967.1	baseplate J/gp47 family protein	-
BCV75_RS06330 (BCV75_05855)	1296619..1296915	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCV75_RS06335 (BCV75_05860)	1296918..1297211	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
BCV75_RS06340 (BCV75_05865)	1297267..1297620	-	354	WP_046419963.1	hypothetical protein	-
BCV75_RS06345 (BCV75_05870)	1297617..1298258	-	642	WP_046419962.1	Gp138 family membrane-puncturing spike protein	-
BCV75_RS06350 (BCV75_05875)	1298255..1299085	-	831	WP_046419672.1	hypothetical protein	-
BCV75_RS06355 (BCV75_05880)	1299082..1299399	-	318	WP_046419669.1	hypothetical protein	-
BCV75_RS06360 (BCV75_05885)	1299399..1300169	-	771	WP_046419667.1	hypothetical protein	-
BCV75_RS06365 (BCV75_05890)	1300577..1300834	-	258	WP_046419904.1	Blp family class II bacteriocin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1286815..1337688	50873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T64844 WP_004085590.1 NZ_CP016608:1296619-1296915 [Xylella fastidiosa]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T64844 NZ_CP016608:1296619-1296915 [Xylella fastidiosa]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCGGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT64844 WP_004085592.1 NZ_CP016608:1296918-1297211 [Xylella fastidiosa]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT64844 NZ_CP016608:1296918-1297211 [Xylella fastidiosa]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAAACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCAACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTAGGGATCGAGCTGTCTGCGAAACCGTCGGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A060HCK8