Detailed information of TA system TA064843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1168403..1168944
• Replicon: chromosome
• Accession: NZ_CP016608
• Organism: Xylella fastidiosa strain Salento-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BCV75_RS05640
• Protein ID: WP_031336866.1
• Coordinates: 1168621..1168944 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BCV75_RS05635
• Protein ID: WP_046419240.1
• Coordinates: 1168403..1168624 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV75_RS05590 (BCV75_05165)	1164638..1164862	-	225	WP_230950005.1	major capsid protein	-
BCV75_RS05595 (BCV75_05170)	1164871..1165077	-	207	WP_046419249.1	hypothetical protein	-
BCV75_RS05605 (BCV75_05175)	1165629..1166216	-	588	WP_046419247.1	hypothetical protein	-
BCV75_RS05615 (BCV75_05180)	1166803..1166985	-	183	WP_046419245.1	hypothetical protein	-
BCV75_RS05620	1166989..1167255	-	267	WP_080939575.1	hypothetical protein	-
BCV75_RS05625 (BCV75_05185)	1167268..1167543	-	276	WP_230950007.1	hypothetical protein	-
BCV75_RS05630 (BCV75_05190)	1167664..1168116	+	453	WP_234048966.1	DUF3693 domain-containing protein	-
BCV75_RS05635 (BCV75_05195)	1168403..1168624	+	222	WP_046419240.1	antitoxin MazE family protein	Antitoxin
BCV75_RS05640 (BCV75_05200)	1168621..1168944	+	324	WP_031336866.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCV75_RS12310	1169034..1169195	-	162	WP_010894327.1	hypothetical protein	-
BCV75_RS05645 (BCV75_05205)	1169202..1169534	-	333	WP_046419237.1	hypothetical protein	-
BCV75_RS05650 (BCV75_05210)	1169521..1170702	-	1182	WP_046419234.1	replication initiation factor domain-containing protein	-
BCV75_RS05665 (BCV75_05220)	1171311..1172003	-	693	WP_023906504.1	7-cyano-7-deazaguanine synthase QueC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1128959..1222311	93352

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11771.81 Da
• Isoelectric Point: 7.9848

>T64843 WP_031336866.1 NZ_CP016608:1168621-1168944 [Xylella fastidiosa]
MIQRGDLVTVSLQGDYGKPRPALIVQSDLLTELDSVALCPVTSDLRNAIFRVTVEPTAANGLRTLSQVMVDKISTLPRNK
ISEPFGRLNDERMKAIERALLLIIGII

Nucleotide

• Length: 324 bp

>T64843 NZ_CP016608:1168621-1168944 [Xylella fastidiosa]
ATGATTCAACGTGGCGACCTTGTAACAGTTTCCCTACAAGGCGACTACGGCAAGCCTAGACCAGCGTTGATCGTTCAATC
AGACCTTCTAACGGAGTTAGATAGTGTCGCACTGTGTCCGGTCACGAGCGACCTGCGGAATGCGATATTTCGTGTCACCG
TTGAACCAACCGCGGCCAATGGTTTACGGACACTGTCGCAGGTCATGGTAGATAAAATTTCAACACTACCACGTAACAAA
ATCAGCGAACCTTTTGGACGTCTCAATGACGAGAGAATGAAAGCAATAGAAAGAGCATTGTTACTAATTATTGGTATCAT
TTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8455.64 Da
• Isoelectric Point: 9.3510

>AT64843 WP_046419240.1 NZ_CP016608:1168403-1168624 [Xylella fastidiosa]
MTSTHRPDSKIVHHREQMRAAGLRPMQLWVPDTRRPEFAAEIQSQCRALKGDQAEADALRFTEKAMTHIEGWK

Nucleotide

• Length: 222 bp

>AT64843 NZ_CP016608:1168403-1168624 [Xylella fastidiosa]
ATGACATCAACTCACAGACCAGACAGCAAGATAGTTCACCATAGGGAACAGATGCGCGCAGCGGGATTGCGTCCTATGCA
GCTCTGGGTGCCGGATACGCGCAGACCGGAATTCGCTGCTGAAATTCAAAGCCAGTGCCGCGCCCTGAAAGGCGACCAGG
CAGAAGCCGACGCTTTACGCTTCACAGAAAAAGCCATGACCCATATTGAAGGTTGGAAATGA