Detailed information of TA system TA064835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2274519..2275497
• Replicon: chromosome
• Accession: NZ_CP016595
• Organism: Bacillus cereus strain K8

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BA204_RS12220
• Protein ID: WP_000624979.1
• Coordinates: 2274519..2275256 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: BA204_RS12225
• Protein ID: WP_000237818.1
• Coordinates: 2275369..2275497 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA204_RS12190	2270135..2270542	+	408	WP_000072493.1	VOC family protein	-
BA204_RS12195	2270564..2270944	+	381	WP_000497672.1	hypothetical protein	-
BA204_RS12200	2271102..2272787	-	1686	WP_033689678.1	alpha-keto acid decarboxylase family protein	-
BA204_RS12205	2272895..2273377	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
BA204_RS12210	2273544..2274281	+	738	WP_000594151.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BA204_RS12220	2274519..2275256	+	738	WP_000624979.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BA204_RS12225	2275369..2275497	+	129	WP_000237818.1	hypothetical protein	Antitoxin
BA204_RS12230	2275570..2275746	+	177	WP_000852627.1	stage II sporulation protein SB	-
BA204_RS12235	2275766..2276155	-	390	WP_000713864.1	YxeA family protein	-
BA204_RS12240	2276367..2277836	+	1470	WP_000287533.1	beta-Ala-His dipeptidase	-
BA204_RS12245	2278081..2278890	+	810	WP_000664542.1	C39 family peptidase	-
BA204_RS12250	2278916..2279518	+	603	WP_000517260.1	hypothetical protein	-
BA204_RS12255	2279759..2280010	-	252	WP_000827561.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28311.04 Da
• Isoelectric Point: 8.2998

>T64835 WP_000624979.1 NZ_CP016595:2274519-2275256 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHMEQLNEQKAVQVENVGVIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T64835 NZ_CP016595:2274519-2275256 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTAGATATTGCATTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATACGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATGGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGGTTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT64835 WP_000237818.1 NZ_CP016595:2275369-2275497 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT64835 NZ_CP016595:2275369-2275497 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5