Detailed information of TA system TA064832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1177484..1178019
• Replicon: chromosome
• Accession: NZ_CP016592
• Organism: Ketogulonicigenium vulgare strain SKV

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: KvSKV_RS06005
• Protein ID: WP_013384044.1
• Coordinates: 1177771..1178019 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: F9Y4N2
• Locus tag: KvSKV_RS06000
• Protein ID: WP_013384043.1
• Coordinates: 1177484..1177774 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KvSKV_RS05960	1172620..1173024	+	405	WP_013384036.1	hypothetical protein	-
KvSKV_RS05965	1173021..1174175	+	1155	WP_013384037.1	hypothetical protein	-
KvSKV_RS05970	1174175..1174438	+	264	WP_013384038.1	hypothetical protein	-
KvSKV_RS05975	1174551..1175180	+	630	WP_044008023.1	TetR/AcrR family transcriptional regulator	-
KvSKV_RS05980	1175180..1175515	+	336	WP_014537641.1	DoxX family protein	-
KvSKV_RS05985	1175663..1175926	+	264	WP_013384040.1	hypothetical protein	-
KvSKV_RS05990	1175926..1177230	+	1305	WP_065739317.1	hypothetical protein	-
KvSKV_RS05995	1177245..1177472	+	228	WP_013384042.1	hypothetical protein	-
KvSKV_RS06000	1177484..1177774	-	291	WP_013384043.1	BrnA antitoxin family protein	Antitoxin
KvSKV_RS06005	1177771..1178019	-	249	WP_013384044.1	BrnT family toxin	Toxin
KvSKV_RS06010	1178116..1178658	+	543	WP_013384045.1	lysozyme	-
KvSKV_RS06015	1178662..1178883	+	222	WP_013384046.1	hypothetical protein	-
KvSKV_RS15650	1178883..1179098	+	216	WP_162467560.1	hypothetical protein	-
KvSKV_RS06025	1179289..1179747	-	459	WP_014537644.1	sigma-70 family RNA polymerase sigma factor	-
KvSKV_RS06030	1180049..1180975	-	927	WP_065739318.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1138541..1178658	40117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-94)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9028.54 Da
• Isoelectric Point: 7.3199

>T64832 WP_013384044.1 NZ_CP016592:c1178019-1177771 [Ketogulonicigenium vulgare]
MMMIVWDEPKRESNLEKHGLDFADLSEEFFLSSVVVPAKNGRHMAIGKLADGTIAVVFATLGTQGLSVISMRHASKKERA
IL

Nucleotide

• Length: 249 bp

>T64832 NZ_CP016592:c1178019-1177771 [Ketogulonicigenium vulgare]
ATGATGATGATTGTCTGGGATGAACCGAAACGCGAAAGCAACCTCGAGAAACACGGGTTAGATTTCGCAGATTTGTCCGA
GGAATTCTTTCTGTCCTCGGTCGTTGTCCCGGCAAAGAACGGGCGTCACATGGCGATAGGGAAGCTTGCCGACGGAACGA
TTGCAGTGGTCTTTGCCACACTTGGAACGCAAGGCCTGTCGGTGATTTCGATGCGCCACGCCAGCAAAAAGGAAAGGGCG
ATCCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10567.07 Da
• Isoelectric Point: 10.4185

>AT64832 WP_013384043.1 NZ_CP016592:c1177774-1177484 [Ketogulonicigenium vulgare]
MKANKLTEKEIQRRIASDPDAPEATDDQLAKAKPFAEAMPQLAENLRRNLGGRPKSDNPKVAVSLRLDQDVIEKFKAAGP
GWQSRMNKALREAAGV

Nucleotide

• Length: 291 bp

>AT64832 NZ_CP016592:c1177774-1177484 [Ketogulonicigenium vulgare]
ATGAAAGCCAACAAGCTAACCGAGAAAGAAATCCAACGCCGGATCGCAAGCGACCCGGACGCACCCGAGGCAACAGATGA
CCAGCTGGCGAAGGCGAAGCCCTTCGCCGAGGCCATGCCCCAACTGGCAGAGAACCTGCGCCGCAACCTTGGCGGCCGCC
CGAAATCCGACAACCCCAAAGTCGCGGTTTCGCTGCGCCTCGATCAAGACGTGATTGAGAAGTTCAAGGCCGCTGGCCCC
GGCTGGCAGTCGCGCATGAACAAAGCCCTGCGCGAAGCTGCGGGGGTATGA

Structures

Antitoxin

• AlphaFold DB: F9Y4N2