Detailed information of TA system TA064831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3350426..3351404
• Replicon: chromosome
• Accession: NZ_CP016588
• Organism: Bacillus thuringiensis strain KNU-07

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: BCM43_RS16965
• Protein ID: WP_000624977.1
• Coordinates: 3350667..3351404 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: BCM43_RS16960
• Protein ID: WP_000237818.1
• Coordinates: 3350426..3350554 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCM43_RS16930	3345913..3346164	+	252	WP_000827560.1	response regulator transcription factor	-
BCM43_RS16935	3346405..3347007	-	603	WP_000517260.1	hypothetical protein	-
BCM43_RS16940	3347033..3347842	-	810	WP_000678509.1	C39 family peptidase	-
BCM43_RS16945	3348088..3349557	-	1470	WP_065704141.1	beta-Ala-His dipeptidase	-
BCM43_RS16950	3349769..3350158	+	390	WP_000713866.1	YxeA family protein	-
BCM43_RS16955	3350177..3350353	-	177	WP_000852629.1	stage II sporulation protein SB	-
BCM43_RS16960	3350426..3350554	-	129	WP_000237818.1	hypothetical protein	Antitoxin
BCM43_RS16965	3350667..3351404	-	738	WP_000624977.1	stage II sporulation protein SA	Toxin
BCM43_RS16970	3351642..3352379	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BCM43_RS16975	3352546..3353028	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
BCM43_RS16980	3353136..3354821	+	1686	WP_058839616.1	alpha-keto acid decarboxylase family protein	-
BCM43_RS16985	3354979..3355359	-	381	WP_000497670.1	methyltransferase	-
BCM43_RS16990	3355381..3355788	-	408	WP_000063589.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T64831 WP_000624977.1 NZ_CP016588:c3351404-3350667 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T64831 NZ_CP016588:c3351404-3350667 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT64831 WP_000237818.1 NZ_CP016588:c3350554-3350426 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT64831 NZ_CP016588:c3350554-3350426 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5