Detailed information of TA system TA064830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yxiD-yxxD/-
• Location: 2748393..2749554
• Replicon: chromosome
• Accession: NZ_CP016588
• Organism: Bacillus thuringiensis strain KNU-07

Toxin (Protein)

• Gene name: yxiD
• Uniprot ID: -
• Locus tag: BCM43_RS14025
• Protein ID: WP_142320269.1
• Coordinates: 2748393..2749118 (+)
• Length: 242 a.a.

Antitoxin (Protein)

• Gene name: yxxD
• Uniprot ID: A0A7V7HY09
• Locus tag: BCM43_RS14030
• Protein ID: WP_000872947.1
• Coordinates: 2749120..2749554 (+)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCM43_RS13995	2743556..2744083	+	528	WP_001278747.1	hypothetical protein	-
BCM43_RS33235	2744180..2744461	+	282	Protein_2716	DUF1911 domain-containing protein	-
BCM43_RS14005	2744734..2745162	+	429	WP_176523752.1	hypothetical protein	-
BCM43_RS14010	2745190..2746437	+	1248	WP_065703816.1	DUF1911 domain-containing protein	-
BCM43_RS14015	2746735..2747379	+	645	WP_065703818.1	hypothetical protein	-
BCM43_RS14020	2747397..2748128	+	732	WP_000942842.1	DUF1911 domain-containing protein	-
BCM43_RS14025	2748393..2749118	+	726	WP_142320269.1	hypothetical protein	Toxin
BCM43_RS14030	2749120..2749554	+	435	WP_000872947.1	SMI1/KNR4 family protein	Antitoxin
BCM43_RS14035	2749971..2750453	+	483	WP_033690240.1	sugar-binding protein	-
BCM43_RS14040	2750459..2750955	+	497	Protein_2724	SMI1/KNR4 family protein	-
BCM43_RS14045	2751386..2751990	+	605	Protein_2725	hypothetical protein	-
BCM43_RS14050	2752200..2753132	+	933	Protein_2726	MoxR family ATPase	-
BCM43_RS14055	2753129..2754238	+	1110	WP_003312283.1	DUF58 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Knr4/Smi1-like domain (16-130)

Sequences

Toxin

Protein

• Length: 242 a.a.
• Molecular weight: 26874.44 Da
• Isoelectric Point: 10.3528

>T64830 WP_142320269.1 NZ_CP016588:2748393-2749118 [Bacillus thuringiensis]
MNNLIKSSIGHITKGAKASSGVSTFANAVKLANELKPTLEMLQSFKRNASYAFSSVGGTIVTKIPQGELLEAFYKFAKSK
DGGKGTGGVGKGVSGAGSKIDGELIRKYVRDIETQTNRKIPKNQMEELKSALRNTEYKKMTSSETKRYRREFNKIKNSLI
DEWEKQTGQKWPTYTEDVISEKTGKILRKKGEPYDAHHIIENSYRGEHEWWNIYPAKFPNEHQAGIHGSGSPGNKLFNGG
K

Nucleotide

• Length: 726 bp

>T64830 NZ_CP016588:2748393-2749118 [Bacillus thuringiensis]
TTGAATAATTTAATTAAATCTTCAATAGGCCATATCACAAAAGGAGCAAAAGCATCAAGTGGAGTAAGTACATTTGCTAA
TGCAGTAAAACTAGCAAATGAATTGAAGCCTACGCTTGAAATGTTACAATCATTTAAGAGAAATGCTTCCTATGCATTCT
CTAGCGTTGGTGGAACTATTGTAACCAAAATACCTCAAGGCGAATTATTAGAAGCTTTTTATAAGTTTGCGAAGTCTAAA
GATGGTGGTAAGGGTACGGGTGGTGTTGGTAAGGGCGTCAGTGGGGCTGGAAGTAAGATTGATGGAGAACTAATTAGAAA
ATATGTACGAGATATTGAAACACAGACAAATAGAAAAATACCAAAAAACCAAATGGAAGAATTAAAAAGTGCATTAAGAA
ATACAGAATATAAAAAGATGACGTCTTCAGAAACAAAGAGATATAGACGAGAGTTTAATAAAATTAAGAATTCATTAATT
GATGAATGGGAAAAACAAACTGGTCAAAAATGGCCAACTTATACAGAAGATGTGATTTCAGAGAAAACAGGAAAAATCTT
AAGAAAAAAAGGAGAACCTTATGATGCTCATCATATAATTGAAAACTCTTATCGTGGGGAACATGAGTGGTGGAATATCT
ATCCTGCTAAATTTCCTAATGAGCATCAAGCTGGTATTCATGGTTCGGGTTCACCAGGAAATAAACTTTTTAATGGAGGT
AAATAA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 17204.32 Da
• Isoelectric Point: 3.9514

>AT64830 WP_000872947.1 NZ_CP016588:2749120-2749554 [Bacillus thuringiensis]
MKYDYLVSNKHNSFYPVALEEIEEVEEVLGLKFPKELKDFFLNVGYGFIKGSKYNINRIMDPYSIRDFRLKQNDYEFFPD
IEVYDDLEEEIIFFEGNETVLISIKLTTEENNGIYYDEFKIADSLEDFLAKISENDLYYMDLID

Nucleotide

• Length: 435 bp

>AT64830 NZ_CP016588:2749120-2749554 [Bacillus thuringiensis]
ATGAAGTATGATTATCTAGTATCAAATAAACATAATAGCTTTTATCCAGTAGCATTAGAAGAGATAGAAGAAGTGGAAGA
GGTATTAGGTTTGAAATTTCCAAAAGAACTAAAAGATTTCTTCTTAAATGTTGGGTATGGCTTTATCAAAGGTTCTAAGT
ACAATATTAATCGTATTATGGATCCTTATTCTATTAGAGATTTTCGATTAAAGCAAAATGATTATGAATTTTTTCCAGAC
ATTGAAGTTTATGATGATCTTGAAGAAGAAATAATCTTTTTTGAAGGTAATGAAACAGTTTTAATTTCAATTAAATTAAC
AACAGAAGAAAATAATGGGATTTATTATGATGAATTTAAAATTGCCGATTCACTAGAAGATTTTCTTGCAAAAATATCAG
AAAATGATTTATATTATATGGATTTAATTGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V7HY09