Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 81982..82624 Replicon chromosome
Accession NZ_CP016588
Organism Bacillus thuringiensis strain KNU-07

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BCM43_RS00390 Protein ID WP_000635965.1
Coordinates 81982..82332 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCM43_RS00395 Protein ID WP_000004570.1
Coordinates 82337..82624 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCM43_RS00380 78918..79376 - 459 WP_000344235.1 SprT family protein -
BCM43_RS30505 79572..79688 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCM43_RS00385 79746..81914 - 2169 WP_065702662.1 RNA-binding transcriptional accessory protein -
BCM43_RS00390 81982..82332 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCM43_RS00395 82337..82624 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCM43_RS00400 82933..84102 - 1170 WP_000390615.1 alanine racemase -
BCM43_RS00405 84220..85170 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
BCM43_RS00410 85327..85686 - 360 WP_000583417.1 holo-ACP synthase -
BCM43_RS00415 85780..86352 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
BCM43_RS00420 86345..87307 - 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T64829 WP_000635965.1 NZ_CP016588:c82332-81982 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T64829 NZ_CP016588:c82332-81982 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT64829 WP_000004570.1 NZ_CP016588:c82624-82337 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT64829 NZ_CP016588:c82624-82337 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References