Detailed information of TA system TA064816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 4459071..4459636
• Replicon: chromosome
• Accession: NZ_CP016559
• Organism: Streptomyces clavuligerus strain F613-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: BB341_RS18075
• Protein ID: WP_003960643.1
• Coordinates: 4459268..4459636 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: BB341_RS18070
• Protein ID: WP_029182939.1
• Coordinates: 4459071..4459268 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB341_RS18055	4456458..4457021	+	564	WP_003954788.1	AAA family ATPase	-
BB341_RS18065	4457532..4459037	+	1506	WP_003954790.1	MFS transporter	-
BB341_RS18070	4459071..4459268	+	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
BB341_RS18075	4459268..4459636	+	369	WP_003960643.1	Fic family protein	Toxin
BB341_RS18080	4459794..4461587	+	1794	WP_003954793.1	DEAD/DEAH box helicase	-
BB341_RS18085	4461889..4462530	+	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
BB341_RS18090	4462995..4463783	+	789	WP_065757152.1	hypothetical protein	-
BB341_RS18095	4463848..4464318	-	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T64816 WP_003960643.1 NZ_CP016559:4459268-4459636 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T64816 NZ_CP016559:4459268-4459636 [Streptomyces clavuligerus]
ATGCACTACCTCACCCTGCCCGAGCTGCTGAACCTCGCGGAGCGGCTGCGGGCGGCCGAGGTGCGCGACTACGGTCTGCT
GGACTCCGCCCTCGCGCGCCCCCAGGCGAGCGTCTTCGGCCAGGACGCCTACCCGGATGTCTGGCAGAAGGCCGCCGCCC
TGATGGAGTCCCTGGCCCGCAACCACGGACTGGTCGACGGCAACAAGCGGATCGCCTGGTATGCCACCTGGGTCTTTCTC
CATGTGAACGGCCATCCGCTGGACCCGGAATTCGACGTGGACGAGGCGGAACGGTTTGTTCTGGACGTCTGCCAGGGGGC
ACTCGACGTCCCCAAGATCGCGTCGGGGCTGCTGCGCTTCGCCCGCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT64816 WP_029182939.1 NZ_CP016559:4459071-4459268 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT64816 NZ_CP016559:4459071-4459268 [Streptomyces clavuligerus]
ATGGCTGGTCTCAATGTGCGATTCACCGAGGAAGAGCTCACCGCCCTGCGGGAGCGGGCCGAAGCGGAGGGCCGCAGTAT
GCAGTCCTTCGCGCACGACGCGGTGATCAGAGCGATCAACGAACGCGCGCGGCTGTTCAACGAGGCCGCCGAGCATGTGC
TGAGGGCGAGCGAGGAGCTGAACCGGAGGCTCGCCTGA

Structures

Toxin

• AlphaFold DB: E2Q583