Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PhRel2-ATphRel2/DUF4065(antitoxin)
Location 2786119..2787356 Replicon chromosome
Accession NZ_CP016552
Organism Geobacillus stearothermophilus strain DSM 458

Toxin (Protein)


Gene name phRel2 Uniprot ID -
Locus tag GS458_RS14780 Protein ID WP_201261350.1
Coordinates 2786871..2787356 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name ATphRel2 Uniprot ID -
Locus tag GS458_RS14775 Protein ID WP_095859863.1
Coordinates 2786119..2786868 (-) Length 250 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS458_RS14750 2781409..2782851 - 1443 WP_095859859.1 phage portal protein -
GS458_RS14755 2782870..2784288 - 1419 WP_095859860.1 phage terminase large subunit -
GS458_RS14760 2784263..2784730 - 468 WP_095859861.1 hypothetical protein -
GS458_RS14765 2785019..2785381 - 363 WP_013144090.1 DUF2513 domain-containing protein -
GS458_RS14775 2786119..2786868 - 750 WP_095859863.1 DUF4065 domain-containing protein Antitoxin
GS458_RS14780 2786871..2787356 - 486 WP_201261350.1 hypothetical protein Toxin
GS458_RS14785 2787439..2788626 - 1188 WP_015864904.1 IS701 family transposase -
GS458_RS14790 2788738..2788974 - 237 WP_201261306.1 hypothetical protein -
GS458_RS14795 2789339..2789524 - 186 WP_095859864.1 hypothetical protein -
GS458_RS14800 2789521..2789937 - 417 WP_095859865.1 transcriptional regulator -
GS458_RS14805 2789934..2790437 - 504 WP_095859866.1 hypothetical protein -
GS458_RS14810 2790447..2790995 - 549 WP_095859867.1 dUTP diphosphatase -
GS458_RS14815 2791014..2791196 - 183 WP_095859868.1 hypothetical protein -
GS458_RS14825 2791660..2792157 - 498 WP_095859869.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2741580..2800731 59151
- flank IS/Tn - - 2787439..2788626 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RelA/SpoT

(30-136)

Antitoxin

Antitoxin SocA-like, Panacea domain

(113-225)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 19392.02 Da        Isoelectric Point: 7.3179

>T64799 WP_201261350.1 NZ_CP016552:c2787356-2786871 [Geobacillus stearothermophilus]
MIKNYVYKLEELVIDIDIEFTYQHLDCRLRVKQNESIVNKLKYYRVGKKDKGLYPLNKCLNDLLGFRVIVDDFDHDCVYF
DTLCENIKTEYKIRKMNASKNGYKATHIYFYGESNTYFPWELQIWNSNDEEQNVRSHRIHKQEYVKWAAIYKNSSEFEGG
V

Download         Length: 486 bp

>T64799 NZ_CP016552:c2787356-2786871 [Geobacillus stearothermophilus]
CTGATAAAGAATTATGTTTATAAGCTGGAAGAATTGGTGATTGATATTGACATTGAATTTACATATCAGCATTTAGATTG
TCGTTTGCGTGTTAAACAAAATGAATCAATTGTGAATAAATTGAAGTATTATCGAGTAGGTAAGAAGGATAAAGGGCTTT
ATCCGTTGAATAAGTGTTTAAATGATCTGTTAGGATTCAGGGTCATTGTTGATGATTTTGATCATGATTGTGTATATTTC
GATACTTTATGTGAAAACATAAAAACTGAATACAAGATTCGTAAGATGAATGCGTCTAAAAATGGATACAAGGCTACACA
CATATATTTTTATGGAGAGAGCAATACATATTTTCCTTGGGAATTGCAAATATGGAACTCAAACGATGAGGAACAAAATG
TGCGTTCCCATCGTATACATAAACAAGAGTATGTAAAATGGGCGGCTATTTATAAAAATTCGAGCGAATTTGAAGGGGGT
GTTTAG

Antitoxin


Download         Length: 250 a.a.        Molecular weight: 28566.98 Da        Isoelectric Point: 6.5612

>AT64799 WP_095859863.1 NZ_CP016552:c2786868-2786119 [Geobacillus stearothermophilus]
MAFHFIAIVSHYAEGKRIGWHYADEEKLDKKIIHEFLAKVKKKCGDVQLGIHKLSTDSVSWDSVVQRDAFFKDVIITSDI
ETFIELVSQDQELTAYDVAKFILSIIPSSHLKLQKLLYFAYAEFLLRTGEKLFKEPILAFKYGPVVESVFHKYKVHGSSI
IDYKEDETVCIYPEELAVTPSFMKIASSEHGLIALDCLIDVFEKYGHLSAQELVYKTHRPGGPWDRVYKPGKNAVITDDL
ILQYHHVIQ

Download         Length: 750 bp

>AT64799 NZ_CP016552:c2786868-2786119 [Geobacillus stearothermophilus]
ATGGCGTTTCATTTTATAGCCATTGTCAGTCATTATGCAGAGGGCAAAAGGATCGGATGGCATTACGCTGACGAGGAAAA
ACTTGATAAAAAAATCATCCATGAGTTTTTGGCAAAAGTGAAGAAAAAGTGTGGAGACGTACAGTTAGGCATTCATAAAT
TATCTACTGATTCTGTATCATGGGATTCTGTAGTTCAGAGGGATGCTTTTTTTAAAGACGTAATTATCACAAGTGACATA
GAAACGTTCATTGAACTGGTTTCACAAGATCAGGAGTTAACCGCATATGACGTGGCAAAATTTATTTTATCAATTATACC
ATCATCGCATTTGAAGTTACAAAAATTATTATACTTTGCATATGCGGAGTTTCTATTGCGTACTGGTGAAAAATTATTTA
AGGAACCGATATTGGCGTTTAAATATGGACCTGTGGTGGAAAGTGTTTTTCATAAATACAAGGTTCATGGCTCTTCTATA
ATTGACTATAAAGAGGACGAAACAGTTTGTATATATCCTGAGGAACTGGCGGTTACCCCTTCTTTCATGAAAATTGCATC
ATCGGAGCATGGTTTGATAGCGTTAGACTGTCTAATAGATGTCTTTGAAAAATATGGGCATTTGAGTGCTCAGGAACTCG
TTTATAAAACACATCGACCAGGTGGACCTTGGGATAGGGTGTATAAACCGGGAAAAAATGCTGTAATAACGGATGATTTG
ATCCTTCAATACCACCATGTAATTCAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References