Detailed information of TA system TA064798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1564962..1565560
• Replicon: chromosome
• Accession: NZ_CP016552
• Organism: Geobacillus stearothermophilus strain DSM 458

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G8N5V4
• Locus tag: GS458_RS08340
• Protein ID: WP_011231143.1
• Coordinates: 1565231..1565560 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7U9J7G7
• Locus tag: GS458_RS08335
• Protein ID: WP_023634676.1
• Coordinates: 1564962..1565231 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS458_RS08285	1560029..1560234	+	206	Protein_1539	MBL fold metallo-hydrolase	-
GS458_RS08290	1560465..1560764	+	300	WP_095859346.1	transporter suffix domain-containing protein	-
GS458_RS08295	1560774..1561571	+	798	WP_095859347.1	SGNH/GDSL hydrolase family protein	-
GS458_RS08300	1561568..1562482	+	915	WP_053532806.1	ABC transporter ATP-binding protein	-
GS458_RS08305	1562475..1563455	+	981	WP_095859348.1	ABC transporter permease	-
GS458_RS08315	1563831..1564273	-	443	Protein_1544	hypothetical protein	-
GS458_RS08335	1564962..1565231	+	270	WP_023634676.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GS458_RS08340	1565231..1565560	+	330	WP_011231143.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GS458_RS08350	1566013..1566818	+	806	Protein_1547	pirin family protein	-
GS458_RS08355	1566914..1567663	+	750	WP_095859349.1	oxygen-insensitive NADPH nitroreductase	-
GS458_RS08360	1567737..1568480	+	744	WP_095859350.1	NAD-dependent protein deacylase	-
GS458_RS08365	1568514..1569185	+	672	Protein_1550	branched-chain amino acid ABC transporter permease	-
GS458_RS08370	1569185..1569787	+	603	Protein_1551	branched-chain amino acid ABC transporter permease	-
GS458_RS18920	1569785..1570128	+	344	Protein_1552	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• SpoVT-AbrB domain (17-61)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12071.86 Da
• Isoelectric Point: 7.1331

>T64798 WP_011231143.1 NZ_CP016552:1565231-1565560 [Geobacillus stearothermophilus]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Nucleotide

• Length: 330 bp

>T64798 NZ_CP016552:1565231-1565560 [Geobacillus stearothermophilus]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGAAGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9960.61 Da
• Isoelectric Point: 7.2050

>AT64798 WP_023634676.1 NZ_CP016552:1564962-1565231 [Geobacillus stearothermophilus]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEVIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Nucleotide

• Length: 270 bp

>AT64798 NZ_CP016552:1564962-1565231 [Geobacillus stearothermophilus]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACAGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGGTGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9EUB7

Antitoxin

• AlphaFold DB: A0A7U9J7G7