Detailed information of TA system TA064797

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 898968..899772
• Replicon: chromosome
• Accession: NZ_CP016552
• Organism: Geobacillus stearothermophilus strain DSM 458

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: GS458_RS04840
• Protein ID: WP_095859068.1
• Coordinates: 898968..899387 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: GS458_RS04845
• Protein ID: WP_031212949.1
• Coordinates: 899377..899772 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS458_RS04820	894058..895482	-	1425	WP_095859065.1	amino acid permease	-
GS458_RS18700	895537..895965	+	429	WP_033015848.1	hypothetical protein	-
GS458_RS04825	896005..897699	+	1695	WP_095859066.1	M3 family oligoendopeptidase	-
GS458_RS04830	897920..898438	+	519	WP_095859067.1	GNAT family N-acetyltransferase	-
GS458_RS18880	898519..898610	+	92	Protein_865	ABC transporter ATP-binding protein	-
GS458_RS04835	898602..898940	+	339	Protein_866	TetR/AcrR family transcriptional regulator	-
GS458_RS04840	898968..899387	-	420	WP_095859068.1	DUF86 domain-containing protein	Toxin
GS458_RS04845	899377..899772	-	396	WP_031212949.1	nucleotidyltransferase domain-containing protein	Antitoxin
GS458_RS04850	899971..900570	-	600	WP_095859069.1	hypothetical protein	-
GS458_RS04860	901323..903458	-	2136	WP_095859070.1	ATP-dependent Clp protease ATP-binding subunit	-
GS458_RS04865	903726..904472	+	747	WP_050368423.1	FixH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (14-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (5-94)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15904.43 Da
• Isoelectric Point: 8.2859

>T64797 WP_095859068.1 NZ_CP016552:c899387-898968 [Geobacillus stearothermophilus]
MKNDVILNKISVIERCLKRIREEYNGDPKNLQNYTKQDSIVLNLQRACEACIDLAMHIVAEQKFGLPQHSRDAFALLEEH
GVISPSISKKMKAMVGFRNIAVHDYQQLNLGILQAIVEHHLDDFKQFTKAILDYAKKNG

Nucleotide

• Length: 420 bp

>T64797 NZ_CP016552:c899387-898968 [Geobacillus stearothermophilus]
ATGAAAAATGATGTCATTTTAAACAAGATCAGCGTGATCGAACGCTGCCTGAAACGAATTCGTGAAGAATATAACGGCGA
TCCAAAAAATTTACAAAATTACACAAAACAAGATTCGATCGTTCTGAATTTACAGCGGGCGTGCGAGGCATGCATCGATT
TGGCCATGCATATCGTGGCCGAGCAGAAATTCGGATTGCCGCAGCATAGCCGCGATGCATTCGCTCTCCTCGAAGAACAT
GGGGTCATCTCCCCTTCCATAAGCAAAAAGATGAAGGCGATGGTCGGATTCCGCAACATCGCCGTTCACGACTATCAACA
ACTGAACCTTGGCATCTTGCAAGCCATCGTCGAACACCATCTTGATGATTTCAAACAATTTACGAAAGCCATCCTCGATT
ATGCTAAGAAAAACGGCTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14797.97 Da
• Isoelectric Point: 5.6982

>AT64797 WP_031212949.1 NZ_CP016552:c899772-899377 [Geobacillus stearothermophilus]
METIIIQTLRPALHPFVIYLFGSAARGAMRPDSDVDIAFVSDGEPHDPYELFRLAGHLADKLGRDVDLVDLRQASTVFQA
QVVSTGKAIDCRDERKRAEFEMKTLKMYVKLNEERAPVLKQITESGSIYEK

Nucleotide

• Length: 396 bp

>AT64797 NZ_CP016552:c899772-899377 [Geobacillus stearothermophilus]
ATGGAGACGATCATCATTCAGACGCTCCGCCCGGCTCTTCACCCGTTCGTCATCTACCTGTTCGGTTCAGCCGCCCGTGG
GGCGATGCGCCCGGACAGTGATGTCGATATCGCCTTTGTCAGCGACGGCGAACCGCATGATCCGTATGAGCTGTTTCGGC
TCGCGGGGCATTTGGCTGACAAGTTAGGGCGAGATGTCGATCTTGTCGATTTGCGCCAAGCCAGCACCGTGTTTCAAGCG
CAAGTCGTCTCGACGGGAAAAGCCATTGATTGCCGCGACGAGCGGAAACGGGCTGAGTTCGAAATGAAAACATTGAAAAT
GTATGTGAAACTAAACGAAGAGAGAGCACCGGTGTTAAAACAAATTACGGAAAGCGGGAGCATATATGAAAAATGA