Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 217294..217930 Replicon chromosome
Accession NZ_CP016552
Organism Geobacillus stearothermophilus strain DSM 458

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag GS458_RS01195 Protein ID WP_003253417.1
Coordinates 217580..217930 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A087LE06
Locus tag GS458_RS01190 Protein ID WP_033010302.1
Coordinates 217294..217575 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS458_RS01170 213546..214163 - 618 WP_033010298.1 rhomboid family intramembrane serine protease -
GS458_RS01175 214282..214671 + 390 WP_033010299.1 holo-ACP synthase -
GS458_RS01180 214751..215770 + 1020 WP_044743921.1 outer membrane lipoprotein carrier protein LolA -
GS458_RS01185 216013..217179 + 1167 WP_095860169.1 alanine racemase -
GS458_RS01190 217294..217575 + 282 WP_033010302.1 hypothetical protein Antitoxin
GS458_RS01195 217580..217930 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GS458_RS01200 218473..220635 + 2163 WP_095858750.1 RNA-binding transcriptional accessory protein -
GS458_RS01205 220693..220806 - 114 WP_095858751.1 cortex morphogenetic protein CmpA -
GS458_RS01210 220900..221418 + 519 WP_047817690.1 SprT family protein -
GS458_RS18815 222263..222802 - 540 WP_201261317.1 IS630 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T64796 WP_003253417.1 NZ_CP016552:217580-217930 [Geobacillus stearothermophilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T64796 NZ_CP016552:217580-217930 [Geobacillus stearothermophilus]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGATCTTTCCCCGGTTGTTGGCTCAGAGCAGGGCGGCGTCCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGCTTCAGTCCAACGGTTATTGTAGCGGCGATCACGGCGCAAATCCAAAAGG
CAAAGCTGCCGACGCATGTCGAGATCGATGCGAAGCGCTACGGGTTTGAGCGGGACTCGGTCATTTTGCTTGAACAAATC
CGTACGATTGATAAGCAGCGGCTGACTGACAAAATCACCCATCTGGATGATGAGATGATGGACAAAGTCGATGAAGCGCT
GCAAATCAGCTTAGGGCTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10683.20 Da        Isoelectric Point: 7.2027

>AT64796 WP_033010302.1 NZ_CP016552:217294-217575 [Geobacillus stearothermophilus]
VSESGATTEIIVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT64796 NZ_CP016552:217294-217575 [Geobacillus stearothermophilus]
GTGTCGGAATCTGGCGCAACAACGGAAATCATCGTTCGTTTGCCGCAGTCGCTGCTGACGGAGCTCGACGTCCTCGTCAA
ACAGGAGAACGGCAATCGCAATGAACTTATTTACCAGGCGACGAAAATGTATATCCGGGAGCGGAAAAAGCGGCAAATTC
GCGAAGCGATGAGACGTGGCTACATGGAAATGGCGAAAATCAATTTATCTATTGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A087LE06

References