64794

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 19782..20247 Replicon plasmid unnamed4
Accession NZ_CP016550
Organism Escherichia coli strain O177:H21

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag BB405_RS27770 Protein ID WP_001230707.1
Coordinates 19782..20033 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag BB405_RS27775 Protein ID WP_000801440.1
Coordinates 20020..20247 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB405_RS27745 15263..15790 - 528 WP_000577043.1 DUF2726 domain-containing protein -
BB405_RS27750 15925..16329 - 405 WP_012421203.1 hypothetical protein -
BB405_RS27755 16342..17208 - 867 WP_001353743.1 replication initiation protein -
BB405_RS32195 18623..19138 + 516 WP_001025395.1 J domain-containing protein -
BB405_RS27765 19243..19785 + 543 WP_001083906.1 hypothetical protein -
BB405_RS27770 19782..20033 + 252 WP_001230707.1 hypothetical protein Toxin
BB405_RS27775 20020..20247 + 228 WP_000801440.1 hypothetical protein Antitoxin
BB405_RS31390 20701..21000 + 300 WP_000650309.1 hypothetical protein -
BB405_RS27785 21091..21441 + 351 WP_000854259.1 hypothetical protein -
BB405_RS27790 21746..21994 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
BB405_RS27795 21991..22338 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
BB405_RS27800 22699..23202 + 504 WP_000781813.1 hypothetical protein -
BB405_RS27805 23206..24435 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
BB405_RS27810 24512..24805 + 294 WP_060598542.1 LuxR family transcriptional regulator -
BB405_RS27815 24802..25017 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49695 49695


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-92)

Antitoxin

Antitoxin ParD

(1-77)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T64794 WP_001230707.1 NZ_CP016550:19782-20033 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T64794 NZ_CP016550:19782-20033 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT64794 WP_000801440.1 NZ_CP016550:20020-20247 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT64794 NZ_CP016550:20020-20247 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References