Detailed information of TA system TA064793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 76617..77218
• Replicon: plasmid unnamed3
• Accession: NZ_CP016549
• Organism: Escherichia coli strain O177:H21

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: BB405_RS27415
• Protein ID: WP_016231379.1
• Coordinates: 76838..77218 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: BB405_RS27410
• Protein ID: WP_001190712.1
• Coordinates: 76617..76838 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB405_RS27400	73646..74881	-	1236	WP_059338147.1	restriction endonuclease subunit S	-
BB405_RS27405	74878..76434	-	1557	WP_016231378.1	type I restriction-modification system subunit M	-
BB405_RS27410	76617..76838	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BB405_RS27415	76838..77218	+	381	WP_016231379.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BB405_RS27420	77223..77402	+	180	WP_000113019.1	hypothetical protein	-
BB405_RS27425	77430..78473	+	1044	WP_016231380.1	DUF968 domain-containing protein	-
BB405_RS27430	78562..79014	+	453	WP_016231381.1	hypothetical protein	-
BB405_RS27435	79100..80293	+	1194	WP_016231382.1	hypothetical protein	-
BB405_RS27440	80293..81777	+	1485	WP_000124150.1	terminase	-
BB405_RS30275	81999..82118	+	120	WP_001376634.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..126046	126046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13616.35 Da
• Isoelectric Point: 5.1514

>T64793 WP_016231379.1 NZ_CP016549:76838-77218 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELVDLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T64793 NZ_CP016549:76838-77218 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGTAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT64793 WP_001190712.1 NZ_CP016549:76617-76838 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT64793 NZ_CP016549:76617-76838 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6