Detailed information of TA system TA064791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 49565..50058
• Replicon: plasmid unnamed1
• Accession: NZ_CP016547
• Organism: Escherichia coli strain O177:H21

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: BB405_RS26210
• Protein ID: WP_001230709.1
• Coordinates: 49565..49819 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: BB405_RS26215
• Protein ID: WP_000151456.1
• Coordinates: 49816..50058 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB405_RS26170	45140..45301	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
BB405_RS26175	45451..46047	+	597	WP_000125165.1	hypothetical protein	-
BB405_RS32815	46065..46349	-	285	WP_001162371.1	hypothetical protein	-
BB405_RS26185	46423..47088	-	666	WP_000532149.1	P-loop NTPase	-
BB405_RS32130	47312..47527	+	216	WP_001672020.1	hypothetical protein	-
BB405_RS26195	47676..48005	+	330	WP_000855119.1	hypothetical protein	-
BB405_RS26200	48408..48923	+	516	WP_001025393.1	J domain-containing protein	-
BB405_RS26205	49077..49568	+	492	WP_065749866.1	hypothetical protein	-
BB405_RS26210	49565..49819	+	255	WP_001230709.1	hypothetical protein	Toxin
BB405_RS26215	49816..50058	+	243	WP_000151456.1	hypothetical protein	Antitoxin
BB405_RS32490	50264..50497	+	234	WP_000553847.1	hypothetical protein	-
BB405_RS26225	50629..50922	+	294	WP_000673428.1	hypothetical protein	-
BB405_RS26230	51339..51521	-	183	WP_024135751.1	hypothetical protein	-
BB405_RS26235	52079..52261	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
BB405_RS26240	52286..52723	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
BB405_RS26245	52853..53179	-	327	WP_000206697.1	hypothetical protein	-
BB405_RS26250	53176..53385	-	210	WP_000951714.1	hypothetical protein	-
BB405_RS26255	53387..53593	-	207	WP_000999175.1	hypothetical protein	-
BB405_RS26260	53590..54039	-	450	WP_000206698.1	hypothetical protein	-
BB405_RS26265	54059..54766	-	708	WP_021503228.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86371	86371
	-	flank	IS/Tn	-	-	43473..44621	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T64791 WP_001230709.1 NZ_CP016547:49565-49819 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T64791 NZ_CP016547:49565-49819 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT64791 WP_000151456.1 NZ_CP016547:49816-50058 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT64791 NZ_CP016547:49816-50058 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5