Detailed information of TA system TA064780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3866155..3866780
• Replicon: chromosome
• Accession: NZ_CP016546
• Organism: Escherichia coli strain O177:H21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BB405_RS19495
• Protein ID: WP_000911330.1
• Coordinates: 3866382..3866780 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BB405_RS19490
• Protein ID: WP_000450524.1
• Coordinates: 3866155..3866382 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB405_RS19460	3861958..3862428	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
BB405_RS19465	3862428..3863000	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
BB405_RS19470	3863146..3864024	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BB405_RS19475	3864041..3865075	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
BB405_RS19485	3865288..3866001	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BB405_RS19490	3866155..3866382	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BB405_RS19495	3866382..3866780	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BB405_RS19500	3866927..3867790	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
BB405_RS19505	3867805..3869820	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
BB405_RS19510	3869894..3870592	+	699	WP_000679812.1	esterase	-
BB405_RS19515	3870673..3870873	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T64780 WP_000911330.1 NZ_CP016546:3866382-3866780 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T64780 NZ_CP016546:3866382-3866780 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT64780 WP_000450524.1 NZ_CP016546:3866155-3866382 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT64780 NZ_CP016546:3866155-3866382 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33