Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2689320..2689951 Replicon chromosome
Accession NZ_CP016546
Organism Escherichia coli strain O177:H21

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag BB405_RS13425 Protein ID WP_001259388.1
Coordinates 2689320..2689595 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BB405_RS13430 Protein ID WP_000593555.1
Coordinates 2689592..2689951 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB405_RS13410 2684324..2685391 + 1068 WP_000361485.1 HlyD family secretion protein -
BB405_RS13415 2685388..2688123 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
BB405_RS13420 2688123..2689247 + 1125 WP_001314210.1 ABC transporter permease -
BB405_RS13425 2689320..2689595 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
BB405_RS13430 2689592..2689951 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BB405_RS13435 2690071..2690472 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BB405_RS13440 2690478..2691284 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
BB405_RS13445 2691281..2692045 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
BB405_RS13450 2692045..2692878 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
BB405_RS13455 2692875..2693819 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T64774 WP_001259388.1 NZ_CP016546:2689320-2689595 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T64774 NZ_CP016546:2689320-2689595 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT64774 WP_000593555.1 NZ_CP016546:2689592-2689951 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT64774 NZ_CP016546:2689592-2689951 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References