Detailed information of TA system TA064768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1657017..1657837
• Replicon: chromosome
• Accession: NZ_CP016546
• Organism: Escherichia coli strain O177:H21

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: BB405_RS08270
• Protein ID: WP_001054379.1
• Coordinates: 1657017..1657274 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: BB405_RS08275
• Protein ID: WP_000123957.1
• Coordinates: 1657286..1657837 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB405_RS08250	1652304..1653410	+	1107	WP_001295733.1	N-acetylneuraminate epimerase	-
BB405_RS08255	1653474..1654454	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
BB405_RS08260	1655037..1656263	-	1227	Protein_1574	helicase YjhR	-
BB405_RS08265	1656341..1656640	+	300	WP_001332034.1	GNAT family N-acetyltransferase	-
BB405_RS08270	1657017..1657274	+	258	WP_001054379.1	YjhX family toxin	Toxin
BB405_RS08275	1657286..1657837	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
BB405_RS08280	1657889..1658635	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
BB405_RS08285	1658763..1659023	+	261	WP_000077646.1	hypothetical protein	-
BB405_RS32645	1659061..1659177	+	117	Protein_1580	VOC family protein	-
BB405_RS08290	1659422..1660543	+	1122	WP_000010833.1	M42 family metallopeptidase	-
BB405_RS08295	1660540..1660818	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
BB405_RS08300	1660830..1662143	+	1314	WP_029402181.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T64768 WP_001054379.1 NZ_CP016546:1657017-1657274 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T64768 NZ_CP016546:1657017-1657274 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT64768 WP_000123957.1 NZ_CP016546:1657286-1657837 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT64768 NZ_CP016546:1657286-1657837 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACTCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030