64767

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1610156..1610732 Replicon chromosome
Accession NZ_CP016546
Organism Escherichia coli strain O177:H21

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag BB405_RS08030 Protein ID WP_001131965.1
Coordinates 1610445..1610732 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag BB405_RS08025 Protein ID WP_019842999.1
Coordinates 1610156..1610458 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB405_RS08000 1605769..1606281 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
BB405_RS08010 1606671..1608821 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
BB405_RS08015 1608952..1609155 + 204 WP_000467859.1 YbdD/YjiX family protein -
BB405_RS08020 1609166..1610122 + 957 WP_001295525.1 GTPase -
BB405_RS08025 1610156..1610458 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
BB405_RS08030 1610445..1610732 - 288 WP_001131965.1 BrnT family toxin Toxin
BB405_RS08035 1610929..1612095 + 1167 WP_000800825.1 restriction endonuclease -
BB405_RS08040 1612159..1613778 + 1620 WP_001029743.1 type I restriction-modification system subunit M -
BB405_RS08045 1613768..1615192 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T64767 WP_001131965.1 NZ_CP016546:c1610732-1610445 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T64767 NZ_CP016546:c1610732-1610445 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT64767 WP_019842999.1 NZ_CP016546:c1610458-1610156 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT64767 NZ_CP016546:c1610458-1610156 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCAATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References