Detailed information of TA system TA064765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1011173..1012058
• Replicon: chromosome
• Accession: NZ_CP016546
• Organism: Escherichia coli strain O177:H21

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: BB405_RS05040
• Protein ID: WP_065749736.1
• Coordinates: 1011468..1012058 (+)
• Length: 197 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BB405_RS05035
• Protein ID: WP_001297137.1
• Coordinates: 1011173..1011484 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB405_RS05010	1006193..1007140	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
BB405_RS05015	1007162..1009153	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BB405_RS05020	1009143..1009757	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BB405_RS05025	1009757..1010086	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BB405_RS05030	1010098..1010988	+	891	WP_000971336.1	heme o synthase	-
BB405_RS05035	1011173..1011484	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BB405_RS05040	1011468..1012058	+	591	WP_065749736.1	GNAT family acetyltransferase	Toxin
BB405_RS05045	1012065..1013000	-	936	WP_001365761.1	sel1 repeat family protein	-
BB405_RS05050	1013408..1014772	+	1365	WP_001000974.1	MFS transporter	-
BB405_RS05055	1014900..1015391	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
BB405_RS05060	1015559..1016470	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
BB405_RS05065	1016433..1017023	+	591	WP_001276309.1	protein deglycase YajL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1011173..1023068	11895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 197 a.a.
• Molecular weight: 22018.99 Da
• Isoelectric Point: 9.7227

>T64765 WP_065749736.1 NZ_CP016546:1011468-1012058 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGFILMLTLPPLIFMLVSALFSFQR
HQMLLVLYLCFWRFSIFSRLRLLSPPARASFASAGG

Nucleotide

• Length: 591 bp

>T64765 NZ_CP016546:1011468-1012058 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAGATTATTATCGCCACCCGC
CAGGGCAAGTTTTGCCTCAGCCGGGGGCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT64765 WP_001297137.1 NZ_CP016546:1011173-1011484 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT64765 NZ_CP016546:1011173-1011484 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3