Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 977098..977716 Replicon chromosome
Accession NZ_CP016546
Organism Escherichia coli strain O177:H21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BB405_RS04865 Protein ID WP_001291435.1
Coordinates 977498..977716 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BB405_RS04860 Protein ID WP_000344800.1
Coordinates 977098..977472 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB405_RS04850 972187..973380 + 1194 WP_065749735.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BB405_RS04855 973403..976552 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BB405_RS04860 977098..977472 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BB405_RS04865 977498..977716 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BB405_RS04870 977887..978438 + 552 WP_000102564.1 maltose O-acetyltransferase -
BB405_RS04875 978554..979024 + 471 WP_000136192.1 YlaC family protein -
BB405_RS04880 979188..980738 + 1551 Protein_928 cyclic-guanylate-specific phosphodiesterase PdeB -
BB405_RS04885 980780..981133 - 354 WP_000878141.1 DUF1428 family protein -
BB405_RS04895 981512..981823 + 312 WP_000409911.1 MGMT family protein -
BB405_RS04900 981854..982426 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T64764 WP_001291435.1 NZ_CP016546:977498-977716 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T64764 NZ_CP016546:977498-977716 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT64764 WP_000344800.1 NZ_CP016546:977098-977472 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT64764 NZ_CP016546:977098-977472 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References