Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 516517..517156 Replicon chromosome
Accession NZ_CP016543
Organism Planococcus donghaensis strain DSM 22276

Toxin (Protein)


Gene name pemK Uniprot ID E7RK71
Locus tag BCM40_RS02690 Protein ID WP_008432415.1
Coordinates 516806..517156 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID E7RK70
Locus tag BCM40_RS02685 Protein ID WP_008432414.1
Coordinates 516517..516801 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCM40_RS02660 512239..512718 + 480 WP_065527266.1 PH domain-containing protein -
BCM40_RS02665 512711..514222 + 1512 WP_065527759.1 PH domain-containing protein -
BCM40_RS02670 514270..514878 - 609 WP_065527265.1 rhomboid family intramembrane serine protease -
BCM40_RS02675 514947..515300 + 354 WP_065527264.1 holo-ACP synthase -
BCM40_RS02680 515316..516473 + 1158 WP_065527263.1 alanine racemase -
BCM40_RS02685 516517..516801 + 285 WP_008432414.1 hypothetical protein Antitoxin
BCM40_RS02690 516806..517156 + 351 WP_008432415.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BCM40_RS02695 517322..518149 + 828 WP_065527262.1 STAS domain-containing protein -
BCM40_RS02700 518146..518511 + 366 WP_186760007.1 STAS domain-containing protein -
BCM40_RS02705 518514..518915 + 402 WP_008432420.1 anti-sigma regulatory factor -
BCM40_RS02710 518928..519929 + 1002 WP_065527261.1 PP2C family protein-serine/threonine phosphatase -
BCM40_RS02715 520026..520355 + 330 WP_065527260.1 anti-sigma factor antagonist -
BCM40_RS02720 520357..520827 + 471 WP_065527259.1 anti-sigma B factor RsbW -
BCM40_RS02725 520805..521587 + 783 WP_008432426.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12880.97 Da        Isoelectric Point: 5.8817

>T64763 WP_008432415.1 NZ_CP016543:516806-517156 [Planococcus donghaensis]
MIVKRGDVFFAELSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQL
RTIDKSRLTDKITQLDDTLMEKVDEALEISVGLVKF

Download         Length: 351 bp

>T64763 NZ_CP016543:516806-517156 [Planococcus donghaensis]
TTGATTGTAAAACGCGGAGATGTTTTTTTTGCGGAATTATCGCCAGTCGTCGGGTCTGAGCAAGGTGGGACCCGCCCGGT
TCTGGTGATTCAAAACGATATAGGGAATCGGTTTAGTCCCACCGTCATTATTGCTGCAATAACGGCGCAAATTCAAAAAG
CTAAATTACCGACTCATGTAGAAATCAATGCTAAGAAATATGGTTTTGAGCGTGATTCTGTTATTTTACTGGAACAACTA
CGAACAATTGACAAGTCAAGATTGACCGACAAAATTACTCAGCTTGACGACACCTTGATGGAAAAAGTGGATGAAGCATT
GGAAATCAGCGTGGGCCTAGTGAAATTTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10689.03 Da        Isoelectric Point: 6.6344

>AT64763 WP_008432414.1 NZ_CP016543:516517-516801 [Planococcus donghaensis]
VYGKSKTKEVVVKMPKQFISELTTHSNEHVQESGDYIYVSTNRVTKNLYDSYHIREAMIKGYVEMSQINLSIACECSHAE
YEAEHTTVRLVSGG

Download         Length: 285 bp

>AT64763 NZ_CP016543:516517-516801 [Planococcus donghaensis]
GTGTACGGTAAGTCAAAGACAAAAGAAGTGGTTGTGAAAATGCCAAAGCAATTTATTTCGGAGTTGACAACTCATTCAAA
TGAGCATGTTCAGGAAAGTGGAGATTATATTTACGTTTCCACAAACCGGGTGACCAAAAATCTATACGACTCATATCATA
TTCGGGAAGCGATGATCAAGGGCTACGTGGAGATGTCACAAATCAATCTATCGATTGCTTGTGAATGTTCTCACGCTGAG
TACGAAGCGGAGCATACGACCGTGCGACTCGTAAGCGGAGGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C7DUM1

Antitoxin

Source ID Structure
AlphaFold DB E7RK70

References