Detailed information of TA system TA064761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 499160..499799
• Replicon: chromosome
• Accession: NZ_CP016540
• Organism: Planococcus versutus strain L10.15

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: E7RK71
• Locus tag: I858_RS02625
• Protein ID: WP_008432415.1
• Coordinates: 499449..499799 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: I858_RS02620
• Protein ID: WP_049695045.1
• Coordinates: 499160..499444 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I858_RS02595	494879..495358	+	480	WP_049695060.1	PH domain-containing protein	-
I858_RS02600	495351..496853	+	1503	WP_049695061.1	PH domain-containing protein	-
I858_RS02605	496913..497521	-	609	WP_049695042.1	rhomboid family intramembrane serine protease	-
I858_RS02610	497590..497943	+	354	WP_049695043.1	holo-ACP synthase	-
I858_RS02615	497959..499116	+	1158	WP_049695044.1	alanine racemase	-
I858_RS02620	499160..499444	+	285	WP_049695045.1	hypothetical protein	Antitoxin
I858_RS02625	499449..499799	+	351	WP_008432415.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I858_RS02630	499962..500792	+	831	WP_049695046.1	STAS domain-containing protein	-
I858_RS02635	500789..501151	+	363	WP_006830185.1	STAS domain-containing protein	-
I858_RS02640	501154..501555	+	402	WP_049695047.1	anti-sigma regulatory factor	-
I858_RS02645	501568..502569	+	1002	WP_049695048.1	PP2C family protein-serine/threonine phosphatase	-
I858_RS02650	502665..502994	+	330	WP_049695049.1	anti-sigma factor antagonist	-
I858_RS02655	502996..503466	+	471	WP_049695050.1	anti-sigma B factor RsbW	-
I858_RS02660	503444..504226	+	783	WP_049695051.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12880.97 Da
• Isoelectric Point: 5.8817

>T64761 WP_008432415.1 NZ_CP016540:499449-499799 [Planococcus versutus]
MIVKRGDVFFAELSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQL
RTIDKSRLTDKITQLDDTLMEKVDEALEISVGLVKF

Nucleotide

• Length: 351 bp

>T64761 NZ_CP016540:499449-499799 [Planococcus versutus]
TTGATTGTAAAACGCGGAGATGTTTTTTTTGCGGAATTGTCACCAGTCGTCGGGTCTGAGCAAGGCGGGACCCGTCCGGT
TTTGGTGATTCAAAATGATATAGGAAATCGTTTTAGTCCCACGGTTATTATTGCTGCCATAACGGCACAAATTCAAAAAG
CTAAATTGCCTACTCATGTTGAAATCAATGCCAAAAAATATGGCTTTGAGCGTGATTCAGTTATTTTGCTAGAACAATTA
CGAACCATAGACAAGTCTCGACTAACGGATAAAATTACACAACTTGATGACACATTGATGGAGAAAGTAGACGAAGCTTT
AGAAATCAGCGTTGGTCTTGTGAAATTTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10692.10 Da
• Isoelectric Point: 6.6344

>AT64761 WP_049695045.1 NZ_CP016540:499160-499444 [Planococcus versutus]
VYGKSKTKEVVVKMPKQFISELTTHSNEHVQESGDYIYVSTSRVTKNLYDSYHIREAMIKGYVEMSQINLSIACECSHAE
YEAEHMTVRLVSGG

Nucleotide

• Length: 285 bp

>AT64761 NZ_CP016540:499160-499444 [Planococcus versutus]
GTGTACGGGAAGTCAAAGACTAAAGAAGTGGTTGTGAAAATGCCAAAGCAATTTATTTCGGAGTTGACAACTCATTCAAA
TGAGCATGTCCAAGAAAGTGGAGATTATATTTATGTTTCCACGAGCCGAGTGACTAAAAATCTGTATGACTCATATCATA
TTCGAGAAGCGATGATCAAAGGCTACGTGGAGATGTCTCAAATCAATCTATCGATTGCTTGTGAATGCTCTCACGCTGAG
TACGAAGCGGAGCACATGACCGTCCGACTCGTAAGCGGAGGTTGA

Structures

Toxin

• AlphaFold DB: A0A1C7DUM1