Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 183840..184420 Replicon plasmid pBSY240_1
Accession NZ_CP016500
Organism Agrobacterium sp. RAC06

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B3P4V3
Locus tag BSY240_RS22860 Protein ID WP_069044232.1
Coordinates 183840..184223 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B3P4Z7
Locus tag BSY240_RS22865 Protein ID WP_069044233.1
Coordinates 184220..184420 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY240_RS22840 179365..180387 + 1023 WP_150127579.1 AraC family transcriptional regulator -
BSY240_RS22845 181155..182393 - 1239 WP_069044229.1 RNA polymerase subunit sigma-70 -
BSY240_RS22850 182393..182752 - 360 WP_069044230.1 hypothetical protein -
BSY240_RS22855 182901..183260 - 360 WP_069044231.1 hypothetical protein -
BSY240_RS22860 183840..184223 - 384 WP_069044232.1 type II toxin-antitoxin system VapC family toxin Toxin
BSY240_RS22865 184220..184420 - 201 WP_069044233.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BSY240_RS22870 184811..186031 - 1221 WP_150127580.1 HAMP domain-containing protein -
BSY240_RS22875 186090..186797 - 708 WP_083229782.1 response regulator -
BSY240_RS22880 186983..187660 + 678 WP_069044235.1 SH3 domain-containing protein -
BSY240_RS22885 187822..188136 + 315 WP_069044236.1 EF-hand domain-containing protein -
BSY240_RS22890 188133..189392 + 1260 WP_069044237.1 efflux RND transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - tviB / bplB / bplC 1..323507 323507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13930.97 Da        Isoelectric Point: 6.2103

>T64744 WP_069044232.1 NZ_CP016500:c184223-183840 [Agrobacterium sp. RAC06]
VILVDTSIWIDHFRYGDAELGKIITDDLLLCHPFVVGELALGSLRERDAVIAFLAAQREATIATHTEVMTVIDRHSIFSM
GIGYTDAHLLTSTLLDGRASLWTRDKRLAAAAQKVGATLYQQAQAAH

Download         Length: 384 bp

>T64744 NZ_CP016500:c184223-183840 [Agrobacterium sp. RAC06]
GTGATCCTGGTCGACACCTCAATCTGGATCGACCATTTTCGATATGGTGACGCAGAACTAGGGAAAATCATCACTGATGA
CCTACTGCTCTGCCACCCTTTTGTTGTCGGCGAACTGGCGCTTGGTAGCCTACGAGAGCGGGATGCCGTCATAGCTTTCC
TAGCCGCTCAGCGCGAAGCAACGATCGCGACCCATACAGAGGTCATGACAGTAATTGATCGGCATTCGATATTCAGCATG
GGGATCGGTTACACAGACGCGCACTTGTTGACTTCAACACTGCTCGACGGGCGCGCAAGCTTGTGGACAAGAGACAAACG
CCTGGCGGCCGCAGCACAAAAAGTTGGTGCGACGCTTTACCAGCAGGCGCAAGCCGCTCATTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7198.26 Da        Isoelectric Point: 10.2947

>AT64744 WP_069044233.1 NZ_CP016500:c184420-184220 [Agrobacterium sp. RAC06]
MRSTINLDDDLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGSMPEAEASPRRRSDVAK

Download         Length: 201 bp

>AT64744 NZ_CP016500:c184420-184220 [Agrobacterium sp. RAC06]
ATGCGATCTACCATAAATCTCGATGACGATCTCATGGAAAGGGCCAAGTCCCTCACGGGCACGAAGGAAACAGCAGCTCT
TGTTCGACAGGCGCTGGAGACCCTTGTGCGTGTGGAATCTGGCAAACGGCTGATCGCCCTTGGTGGCTCAATGCCAGAGG
CTGAGGCGTCACCGCGCCGACGGAGCGACGTAGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3P4V3

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3P4Z7

References