Detailed information of TA system TA064743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 71843..73329
• Replicon: plasmid pBSY240_1
• Accession: NZ_CP016500
• Organism: Agrobacterium sp. RAC06

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A1B3P5E9
• Locus tag: BSY240_RS22360
• Protein ID: WP_069044144.1
• Coordinates: 72067..73329 (+)
• Length: 421 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: BSY240_RS22355
• Protein ID: WP_083229771.1
• Coordinates: 71843..72070 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSY240_RS22335	66962..68401	+	1440	WP_069044142.1	aldehyde dehydrogenase family protein	-
BSY240_RS22340	68582..69043	+	462	Protein_65	transposase	-
BSY240_RS22345	69329..70300	+	972	WP_083229798.1	NAD(P)H-binding protein	-
BSY240_RS22350	70436..71359	+	924	WP_150127590.1	alpha/beta hydrolase	-
BSY240_RS22355	71843..72070	+	228	WP_083229771.1	helix-turn-helix transcriptional regulator	Antitoxin
BSY240_RS22360	72067..73329	+	1263	WP_069044144.1	type II toxin-antitoxin system HipA family toxin	Toxin
BSY240_RS22365	73538..75340	-	1803	WP_069044145.1	NAD(P)/FAD-dependent oxidoreductase	-
BSY240_RS22370	75459..76532	-	1074	WP_069044146.1	2,3-butanediol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	tviB / bplB / bplC	1..323507	323507
	-	flank	IS/Tn	-	-	68582..69100	518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-370)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-48)

Sequences

Toxin

Protein

• Length: 421 a.a.
• Molecular weight: 45830.71 Da
• Isoelectric Point: 5.5201

>T64743 WP_069044144.1 NZ_CP016500:72067-73329 [Agrobacterium sp. RAC06]
MTRVLDVYFRDTKAGVLGRLDDGLLTFAYDGAYLLDQRSRAISFSMPLQEKPFGDQVVRPFFSGLLPDEGARQRLAGALG
LSAGNAFGLLEIIGGECAGALSLYPPGTAPEPPGQSQAEILTSERLDEILSRLRTRPLLGGDEGIRLSLAGAQDKLAVIV
DGDSIALARDGRPTTHILKPLIQALEGTVENEYFCMRLGARLKLPVPHVEIRLDGATAFLLVERYDRTKAADGIIERLHQ
EDFCQALSVPPELKYEAEGGPGTERALGLIDQVCARPAADRLQFIRMLIFHYLVGNADAHGKNYALLYGSDEPDLAPIYD
VVCTAVYPRLSKRLAMKIGGRDVPDTIQMKHWTTLVPETKSSQRILVRDLAQMAERILSEADALVAELADEGIRHPILSA
IRKVISTRAGLLQKAAEQVS

Nucleotide

• Length: 1263 bp

>T64743 NZ_CP016500:72067-73329 [Agrobacterium sp. RAC06]
ATGACACGCGTCTTGGACGTCTATTTTCGCGACACCAAGGCGGGCGTACTCGGCCGGCTTGATGACGGCCTGCTGACTTT
TGCCTATGACGGCGCGTATCTCCTGGACCAACGATCGCGTGCGATCTCGTTCTCGATGCCCCTGCAAGAAAAGCCATTTG
GTGATCAGGTGGTGCGCCCGTTTTTCTCCGGCCTCTTGCCGGACGAAGGCGCACGGCAACGCCTAGCCGGCGCGCTCGGC
CTTTCGGCAGGCAATGCTTTCGGGCTACTGGAAATTATCGGGGGTGAATGCGCAGGTGCCTTGTCACTCTATCCCCCCGG
CACGGCGCCCGAACCTCCCGGCCAGTCCCAAGCCGAAATTCTCACTTCGGAACGTCTGGATGAAATTCTCAGCAGGTTGC
GGACCCGCCCTTTGCTTGGTGGAGACGAGGGCATTCGCTTATCGCTCGCCGGCGCGCAGGATAAACTGGCGGTCATTGTC
GATGGAGATTCCATTGCCTTGGCGAGAGATGGTCGACCAACGACCCATATCTTGAAACCGCTCATCCAGGCGTTGGAAGG
CACGGTGGAGAACGAGTATTTTTGTATGCGGCTGGGTGCCCGCCTCAAGCTGCCGGTACCCCATGTCGAGATACGCCTAG
ACGGGGCGACTGCCTTTCTCCTGGTTGAACGTTATGATCGGACGAAGGCTGCAGACGGCATCATTGAGCGCCTACACCAG
GAAGACTTTTGCCAGGCCCTTAGCGTACCGCCGGAGCTGAAGTATGAGGCCGAGGGCGGACCGGGAACAGAACGGGCTCT
CGGGCTGATCGACCAGGTCTGCGCTAGACCTGCCGCGGATCGGTTACAGTTCATCCGCATGCTGATCTTCCACTATCTCG
TCGGCAACGCCGATGCCCACGGCAAGAACTACGCCCTGCTTTACGGCTCCGATGAACCGGATCTGGCACCGATCTACGAT
GTCGTTTGCACGGCCGTTTATCCTCGCTTGTCAAAGAGGCTCGCCATGAAGATTGGCGGCCGCGATGTGCCCGACACGAT
CCAGATGAAGCATTGGACGACATTGGTGCCCGAAACCAAATCTTCTCAGCGCATCCTTGTTCGCGATCTTGCCCAAATGG
CCGAAAGGATTTTGAGCGAGGCCGATGCCCTTGTTGCAGAACTGGCCGACGAGGGGATCAGGCACCCCATCCTGAGCGCA
ATTCGCAAGGTCATCTCTACTCGGGCTGGTTTGCTACAGAAAGCCGCGGAGCAGGTGTCTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8151.52 Da
• Isoelectric Point: 10.7429

>AT64743 WP_083229771.1 NZ_CP016500:71843-72070 [Agrobacterium sp. RAC06]
METIKDTKSLGMLIREERKQQGLTQEQLAGVIGVGVRFVRELEAGKESCQIGRAMQVLAGLGLVVSVGRRQGPRS

Nucleotide

• Length: 228 bp

>AT64743 NZ_CP016500:71843-72070 [Agrobacterium sp. RAC06]
ATGGAAACGATCAAAGATACGAAAAGCCTCGGAATGCTGATCCGTGAGGAGCGCAAACAGCAGGGTTTAACCCAGGAGCA
ACTCGCCGGGGTGATTGGTGTGGGCGTACGGTTTGTGCGTGAACTCGAGGCTGGCAAAGAGAGCTGCCAGATTGGCCGTG
CGATGCAAGTCTTGGCTGGTTTGGGCCTCGTTGTTTCGGTCGGGCGTCGCCAGGGCCCGCGATCATGA

Structures

Toxin

• AlphaFold DB: A0A1B3P5E9