Detailed information of TA system TA064738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1659792..1660419
• Replicon: chromosome
• Accession: NZ_CP016499
• Organism: Agrobacterium sp. RAC06

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BSY240_RS23665
• Protein ID: WP_082347610.1
• Coordinates: 1660231..1660419 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0N1L6K4
• Locus tag: BSY240_RS08110
• Protein ID: WP_054148955.1
• Coordinates: 1659792..1660226 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSY240_RS08075	1655187..1655525	+	339	WP_083229725.1	hypothetical protein	-
BSY240_RS08080	1655556..1656161	-	606	WP_069041963.1	YqgE/AlgH family protein	-
BSY240_RS08085	1656337..1657197	+	861	WP_069043893.1	hypothetical protein	-
BSY240_RS08090	1657269..1657760	+	492	WP_069043894.1	peroxiredoxin	-
BSY240_RS08095	1657828..1658136	-	309	WP_054148958.1	Dabb family protein	-
BSY240_RS08100	1658229..1658699	-	471	WP_054148957.1	ribonuclease HI	-
BSY240_RS08105	1658696..1659661	-	966	WP_069041964.1	homoserine kinase	-
BSY240_RS08110	1659792..1660226	-	435	WP_054148955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BSY240_RS23665	1660231..1660419	-	189	WP_082347610.1	type II toxin-antitoxin system HicA family toxin	Toxin
BSY240_RS08120	1660571..1662250	+	1680	WP_069043895.1	EAL domain-containing protein	-
BSY240_RS24125	1662265..1662432	-	168	WP_171901559.1	hypothetical protein	-
BSY240_RS08125	1662510..1663514	-	1005	WP_054148952.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BSY240_RS08130	1663519..1663884	-	366	WP_054148951.1	DUF1304 domain-containing protein	-
BSY240_RS08135	1663957..1664700	-	744	WP_054148950.1	SURF1 family protein	-
BSY240_RS08140	1664708..1665091	-	384	WP_054148949.1	DUF983 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6951.12 Da
• Isoelectric Point: 11.0889

>T64738 WP_082347610.1 NZ_CP016499:c1660419-1660231 [Agrobacterium sp. RAC06]
MRSSAVIAALKADGWYEVSTKGSHVQFKHEKKPGRVTVPHPKRDLPIGTLKNIEKQSSLKLR

Nucleotide

• Length: 189 bp

>T64738 NZ_CP016499:c1660419-1660231 [Agrobacterium sp. RAC06]
ATGAGGAGTTCAGCAGTTATCGCCGCGTTGAAAGCTGACGGGTGGTATGAAGTCTCCACCAAGGGCAGTCATGTGCAGTT
CAAGCATGAGAAGAAGCCCGGCCGCGTCACTGTCCCTCATCCGAAGAGAGATCTGCCAATCGGCACCCTGAAAAATATCG
AGAAACAGTCTTCGTTGAAGCTGAGGTGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 15358.14 Da
• Isoelectric Point: 4.2760

>AT64738 WP_054148955.1 NZ_CP016499:c1660226-1659792 [Agrobacterium sp. RAC06]
MQNFIGLIHKDPDSDYGVSFPDFPGVATAGTDLDDARRMAEEALAFHVEGMIEDGEAIPEPSSLEAIMSDPGNSDAVAVL
IPLRTPAKKAVRVNITLAEDLLRAIDAHAEAHGLTRSGFLARAAEQQMTSDNNPNNIRGYALSA

Nucleotide

• Length: 435 bp

>AT64738 NZ_CP016499:c1660226-1659792 [Agrobacterium sp. RAC06]
ATGCAGAATTTCATTGGCCTCATTCACAAGGATCCCGACAGCGATTACGGCGTCTCCTTCCCCGATTTCCCCGGTGTCGC
CACGGCGGGTACGGATCTGGACGATGCACGCCGCATGGCGGAGGAAGCTCTGGCATTCCATGTCGAAGGCATGATTGAGG
ATGGTGAGGCCATTCCGGAGCCTTCGTCGCTCGAGGCAATCATGTCTGATCCTGGAAATAGCGATGCCGTGGCCGTTCTC
ATCCCGCTGAGAACCCCTGCGAAGAAGGCAGTCCGCGTCAACATCACTCTGGCCGAAGACCTCCTGCGTGCCATTGACGC
TCATGCCGAGGCGCATGGTCTGACGCGCTCTGGTTTCTTGGCCAGGGCGGCAGAGCAGCAGATGACGAGCGACAACAATC
CGAACAACATCCGGGGCTATGCGCTCTCAGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1L6K4