Detailed information of TA system TA064737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1492363..1493144
• Replicon: chromosome
• Accession: NZ_CP016499
• Organism: Agrobacterium sp. RAC06

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A1B3NTY1
• Locus tag: BSY240_RS07240
• Protein ID: WP_069041854.1
• Coordinates: 1492626..1493144 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: BSY240_RS07235
• Protein ID: WP_210183773.1
• Coordinates: 1492363..1492623 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSY240_RS07210	1487539..1488018	+	480	WP_054151094.1	ABZJ_00895 family protein	-
BSY240_RS07215	1488175..1488834	-	660	WP_054151095.1	hemolysin III family protein	-
BSY240_RS07220	1489035..1489418	+	384	WP_054151096.1	MAPEG family protein	-
BSY240_RS07225	1489779..1491590	+	1812	WP_069041851.1	methyl-accepting chemotaxis protein	-
BSY240_RS07230	1491630..1492136	-	507	WP_069041852.1	DUF305 domain-containing protein	-
BSY240_RS07235	1492363..1492623	+	261	WP_210183773.1	DUF1778 domain-containing protein	Antitoxin
BSY240_RS07240	1492626..1493144	+	519	WP_069041854.1	GNAT family N-acetyltransferase	Toxin
BSY240_RS07245	1493228..1494013	-	786	WP_054151101.1	sugar ABC transporter ATP-binding protein	-
BSY240_RS07250	1494028..1495341	-	1314	WP_054151102.1	sugar ABC transporter permease	-
BSY240_RS07255	1495587..1496627	-	1041	WP_054151103.1	D-xylose ABC transporter substrate-binding protein	-
BSY240_RS07260	1496867..1498078	+	1212	WP_069043874.1	ROK family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (33-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-74)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18389.15 Da
• Isoelectric Point: 8.3164

>T64737 WP_069041854.1 NZ_CP016499:1492626-1493144 [Agrobacterium sp. RAC06]
MALSAPEPLDDAHDLSAFSCGKPALDIWLKTRARANQENGFTVVIVVHDAGRIVGYYGHSPTAALPASLPRPIRTGQPPN
PVPCLLLGQLAVDQSYAGHGIGTGLIKHALTRCVQGAALIGGRALIVNAIDEEARLFWTRRGFLPSKDDPFRLLRPISHI
AAALEATEKHHG

Nucleotide

• Length: 519 bp

>T64737 NZ_CP016499:1492626-1493144 [Agrobacterium sp. RAC06]
ATGGCGCTTTCCGCTCCCGAGCCGCTCGATGATGCACACGATCTGAGCGCCTTTTCCTGTGGCAAGCCTGCGCTCGACAT
CTGGCTGAAAACACGGGCGCGTGCCAATCAGGAGAACGGCTTTACCGTCGTGATCGTGGTGCATGATGCAGGCCGCATCG
TCGGCTATTACGGCCACTCGCCGACGGCCGCGCTGCCGGCGTCCCTGCCCCGCCCGATCCGCACCGGCCAGCCGCCCAAC
CCCGTCCCATGCCTGCTGCTCGGGCAGCTTGCCGTCGATCAATCCTATGCCGGGCATGGGATCGGGACAGGTCTGATCAA
ACATGCGCTTACCCGCTGCGTGCAGGGCGCAGCGCTTATTGGCGGGCGGGCGCTGATCGTCAATGCGATTGATGAGGAGG
CGCGGCTGTTCTGGACGCGGCGTGGATTTCTGCCGTCGAAAGATGATCCGTTTCGGCTGTTGCGGCCGATCTCGCATATT
GCGGCGGCGCTTGAGGCGACGGAAAAGCATCACGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9636.16 Da
• Isoelectric Point: 4.5230

>AT64737 WP_210183773.1 NZ_CP016499:1492363-1492623 [Agrobacterium sp. RAC06]
MRLPEADVAMIDRAAALKGRSRTDFVRDAAVRAAEEALMEQQLIRMTEEGFADFMALMAAPVEVVPELVEVMRRPAPWEA
GYRAKD

Nucleotide

• Length: 261 bp

>AT64737 NZ_CP016499:1492363-1492623 [Agrobacterium sp. RAC06]
ATGCGCCTGCCGGAAGCGGATGTGGCGATGATCGACCGCGCGGCGGCTCTTAAAGGCCGGTCGCGGACAGACTTCGTCCG
CGATGCGGCCGTGCGCGCCGCCGAAGAAGCACTCATGGAACAACAGCTAATCCGGATGACGGAGGAAGGCTTTGCCGACT
TCATGGCGCTGATGGCGGCGCCGGTGGAGGTTGTGCCTGAACTGGTTGAGGTCATGCGCCGCCCTGCCCCCTGGGAGGCG
GGTTACAGAGCGAAGGACTGA

Structures

Toxin

• AlphaFold DB: A0A1B3NTY1