Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 139624..140159 Replicon plasmid unnamed1
Accession NZ_CP016498
Organism Escherichia coli strain UPEC 26-1

Toxin (Protein)


Gene name relE Uniprot ID A0A0M1UJC2
Locus tag BB344_RS26370 Protein ID WP_001270422.1
Coordinates 139872..140159 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag BB344_RS26365 Protein ID WP_001132895.1
Coordinates 139624..139875 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB344_RS29490 134642..134953 - 312 WP_001309256.1 hypothetical protein -
BB344_RS30095 135017..135376 - 360 WP_001336695.1 DUF1281 domain-containing protein -
BB344_RS30100 135383..135526 - 144 WP_000270807.1 DUF1281 domain-containing protein -
BB344_RS28665 135692..136468 - 777 Protein_157 hypothetical protein -
BB344_RS26350 136702..137664 - 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
BB344_RS26355 137661..138866 - 1206 WP_000817635.1 AAA family ATPase -
BB344_RS26365 139624..139875 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BB344_RS26370 139872..140159 + 288 WP_001270422.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB344_RS28675 140161..140572 + 412 Protein_162 DDE-type integrase/transposase/recombinase -
BB344_RS26385 141216..142193 + 978 WP_001309253.1 RepB family plasmid replication initiator protein -
BB344_RS26390 142436..143176 - 741 WP_001066947.1 tyrosine-type recombinase/integrase -
BB344_RS26395 143297..143485 - 189 WP_001332784.1 hypothetical protein -
BB344_RS26400 143852..145021 + 1170 WP_001072358.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A) senB 1..151033 151033
- flank IS/Tn - - 135905..136045 140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.30 Da        Isoelectric Point: 10.4703

>T64735 WP_001270422.1 NZ_CP016498:139872-140159 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T64735 NZ_CP016498:139872-140159 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT64735 WP_001132895.1 NZ_CP016498:139624-139875 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT64735 NZ_CP016498:139624-139875 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M1UJC2

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References