Detailed information of TA system TA064724

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4638787..4639619
• Replicon: chromosome
• Accession: NZ_CP016497
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A426P9T8
• Locus tag: BB344_RS22770
• Protein ID: WP_001555674.1
• Coordinates: 4638787..4639161 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A085P7I7
• Locus tag: BB344_RS22775
• Protein ID: WP_001327592.1
• Coordinates: 4639251..4639619 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS22745	4634849..4635832	-	984	WP_001336760.1	KpsF/GutQ family sugar-phosphate isomerase	-
BB344_RS22750	4636643..4636813	-	171	Protein_4324	IS110 family transposase	-
BB344_RS22755	4637155..4637997	-	843	WP_001519083.1	DUF4942 domain-containing protein	-
BB344_RS22760	4638082..4638279	-	198	WP_000445282.1	DUF957 domain-containing protein	-
BB344_RS22765	4638299..4638790	-	492	WP_001555675.1	hypothetical protein	-
BB344_RS22770	4638787..4639161	-	375	WP_001555674.1	TA system toxin CbtA family protein	Toxin
BB344_RS22775	4639251..4639619	-	369	WP_001327592.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BB344_RS22785	4639782..4640003	-	222	WP_000692323.1	DUF987 domain-containing protein	-
BB344_RS22790	4640066..4640542	-	477	WP_001186727.1	RadC family protein	-
BB344_RS22795	4640558..4641037	-	480	WP_000706981.1	antirestriction protein	-
BB344_RS22805	4641376..4642194	-	819	WP_001555673.1	DUF945 domain-containing protein	-
BB344_RS22810	4642284..4642517	-	234	WP_001278282.1	DUF905 family protein	-
BB344_RS22815	4642523..4643200	-	678	WP_001555672.1	hypothetical protein	-
BB344_RS22820	4643348..4644028	-	681	WP_001555671.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14205.16 Da
• Isoelectric Point: 8.2918

>T64724 WP_001555674.1 NZ_CP016497:c4639161-4638787 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAK

Nucleotide

• Length: 375 bp

>T64724 NZ_CP016497:c4639161-4638787 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13901.65 Da
• Isoelectric Point: 6.3165

>AT64724 WP_001327592.1 NZ_CP016497:c4639619-4639251 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT64724 NZ_CP016497:c4639619-4639251 [Escherichia coli]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGACTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A426P9T8

Antitoxin

• AlphaFold DB: A0A085P7I7