Detailed information of TA system TA064722

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 3847078..3847869
• Replicon: chromosome
• Accession: NZ_CP016497
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: BB344_RS18875
• Protein ID: WP_000691791.1
• Coordinates: 3847078..3847503 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: BB344_RS18880
• Protein ID: WP_000939437.1
• Coordinates: 3847534..3847869 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS18845	3842744..3843241	-	498	WP_000509808.1	hypothetical protein	-
BB344_RS18855	3843923..3844309	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
BB344_RS18865	3844932..3845663	-	732	WP_001216639.1	TIGR02646 family protein	-
BB344_RS29900	3845660..3845881	-	222	WP_125112077.1	hypothetical protein	-
BB344_RS18870	3845866..3847057	-	1192	Protein_3578	AAA family ATPase	-
BB344_RS18875	3847078..3847503	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
BB344_RS18880	3847534..3847869	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BB344_RS18885	3847869..3848342	-	474	WP_001292742.1	DNA repair protein RadC	-
BB344_RS18890	3848372..3849186	-	815	Protein_3582	DUF945 domain-containing protein	-
BB344_RS18895	3849422..3850375	-	954	WP_000290406.1	hypothetical protein	-
BB344_RS18900	3850968..3851582	+	615	WP_000772910.1	inovirus Gp2 family protein	-
BB344_RS18905	3851700..3851918	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3844932..3868863	23931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T64722 WP_000691791.1 NZ_CP016497:c3847503-3847078 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T64722 NZ_CP016497:c3847503-3847078 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT64722 WP_000939437.1 NZ_CP016497:c3847869-3847534 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT64722 NZ_CP016497:c3847869-3847534 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6