Detailed information of TA system TA064694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3503956..3504481
• Replicon: chromosome
• Accession: NZ_CP016490
• Organism: Halomonas sp. GFAJ-1 strain Halomonas sp. GFAJ-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H0J4P0
• Locus tag: BB497_RS15915
• Protein ID: WP_009100486.1
• Coordinates: 3503956..3504240 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H0J4N9
• Locus tag: BB497_RS15920
• Protein ID: WP_009100484.1
• Coordinates: 3504230..3504481 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB497_RS15880	3499243..3500160	-	918	WP_009100501.1	restriction endonuclease	-
BB497_RS15885	3500238..3500495	-	258	WP_009100500.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BB497_RS15890	3500530..3500865	-	336	WP_009100498.1	PhnA domain-containing protein	-
BB497_RS15895	3500964..3501530	-	567	WP_009100496.1	pyridoxamine 5'-phosphate oxidase family protein	-
BB497_RS15900	3501705..3502673	+	969	WP_009100494.1	putative zinc-binding metallopeptidase	-
BB497_RS15905	3502679..3503571	-	893	Protein_3088	dihydrodipicolinate synthase family protein	-
BB497_RS15910	3503593..3503913	-	321	WP_009100489.1	hypothetical protein	-
BB497_RS15915	3503956..3504240	-	285	WP_009100486.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BB497_RS15920	3504230..3504481	-	252	WP_009100484.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BB497_RS15925	3504594..3505412	-	819	WP_009100483.1	tryptophan synthase subunit alpha	-
BB497_RS15930	3505469..3506686	-	1218	WP_009100481.1	tryptophan synthase subunit beta	-
BB497_RS15935	3506810..3507703	+	894	WP_009100478.1	LysR family transcriptional regulator	-
BB497_RS15940	3507730..3508332	-	603	WP_009100476.1	glutathione S-transferase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-76)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11028.07 Da
• Isoelectric Point: 10.4268

>T64694 WP_009100486.1 NZ_CP016490:c3504240-3503956 [Halomonas sp. GFAJ-1]
MTYKLRFLPAALKEWEKLSPPVKQQFKKKLAERLETPRVPADKLSGFDSCYKIKLRSVGYRLVYEVVDDELFIYVIAVGK
RDKGNVYASLKRRM

Nucleotide

• Length: 285 bp

>T64694 NZ_CP016490:c3504240-3503956 [Halomonas sp. GFAJ-1]
ATGACTTATAAGCTACGCTTTTTGCCCGCGGCCTTGAAAGAGTGGGAAAAGCTTAGCCCACCCGTCAAACAACAGTTCAA
AAAGAAGCTAGCTGAAAGGCTAGAAACTCCACGTGTGCCTGCCGATAAACTCAGTGGTTTCGATTCCTGCTACAAAATAA
AGCTTCGAAGCGTAGGCTATCGTCTGGTTTATGAAGTAGTGGATGATGAACTGTTTATCTACGTTATTGCTGTGGGTAAG
CGTGACAAAGGCAACGTCTACGCGTCGCTAAAGCGCCGGATGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9173.54 Da
• Isoelectric Point: 4.5659

>AT64694 WP_009100484.1 NZ_CP016490:c3504481-3504230 [Halomonas sp. GFAJ-1]
MHQVLANFSVSISELKKNPSALLTQASGAPIAVLNHNKPAAYLVPVETYEAMMEMLEDYELAKLVEERRADKDKAVTVSL
DDL

Nucleotide

• Length: 252 bp

>AT64694 NZ_CP016490:c3504481-3504230 [Halomonas sp. GFAJ-1]
ATGCACCAAGTATTAGCTAACTTTTCGGTCAGTATTTCTGAGCTAAAGAAAAACCCTTCGGCACTGCTGACCCAAGCAAG
CGGTGCCCCCATTGCTGTGTTGAATCACAACAAGCCCGCCGCTTACCTAGTCCCTGTAGAAACTTACGAGGCGATGATGG
AGATGCTGGAAGACTACGAGCTGGCCAAGCTAGTGGAGGAGCGTCGCGCCGATAAAGACAAGGCGGTCACGGTATCACTG
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: H0J4P0

Antitoxin

• AlphaFold DB: H0J4N9