Detailed information of TA system TA064693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/couple_hipB(antitoxin)
• Location: 1838753..1839306
• Replicon: chromosome
• Accession: NZ_CP016490
• Organism: Halomonas sp. GFAJ-1 strain Halomonas sp. GFAJ-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BB497_RS08490
• Protein ID: WP_193391960.1
• Coordinates: 1838753..1838899 (+)
• Length: 49 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BB497_RS08495
• Protein ID: WP_009098849.1
• Coordinates: 1838944..1839306 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB497_RS08475	1834049..1835095	-	1047	WP_105208858.1	tRNA dihydrouridine(20/20a) synthase DusA	-
BB497_RS08480	1836585..1837738	+	1154	WP_105208808.1	IS3 family transposase	-
BB497_RS08485	1837831..1838427	+	597	WP_009098850.1	lipoprotein	-
BB497_RS08490	1838753..1838899	+	147	WP_193391960.1	hypothetical protein	Toxin
BB497_RS08495	1838944..1839306	+	363	WP_009098849.1	helix-turn-helix transcriptional regulator	Antitoxin
BB497_RS08500	1839681..1840262	+	582	WP_009098846.1	hypothetical protein	-
BB497_RS08505	1840283..1840807	-	525	WP_009098845.1	helix-turn-helix domain-containing protein	-
BB497_RS08510	1841106..1842584	+	1479	WP_009098843.1	glutamate--tRNA ligase	-
BB497_RS08525	1843257..1844138	-	882	WP_009098842.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (65-119)

Sequences

Toxin

Protein

• Length: 49 a.a.
• Molecular weight: 5336.16 Da
• Isoelectric Point: 10.1026

>T64693 WP_193391960.1 NZ_CP016490:1838753-1838899 [Halomonas sp. GFAJ-1]
VKAITHRKSALKGSEYIRLRVGDWRVTIDERGDVLDIINIGARGGVCQ

Nucleotide

• Length: 147 bp

>T64693 NZ_CP016490:1838753-1838899 [Halomonas sp. GFAJ-1]
GTGAAAGCGATTACTCACCGTAAAAGCGCGTTGAAGGGTAGCGAGTATATTCGCCTGCGGGTAGGTGACTGGAGGGTGAC
CATCGATGAGCGCGGCGATGTGCTGGATATCATCAACATCGGTGCTCGTGGCGGTGTTTGCCAATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 12990.71 Da
• Isoelectric Point: 4.1778

>AT64693 WP_009098849.1 NZ_CP016490:1838944-1839306 [Halomonas sp. GFAJ-1]
MSVPQIIQTPTGERLVVLPEQEYYALLARLEDQDDIDAANALVARGEESFPEVVVDALLEGVSPVKVYREYRGLRAGELA
EKAGISQGYLSEIESGKKSGSLSVLKRIAAALDVELSDLT

Nucleotide

• Length: 363 bp

>AT64693 NZ_CP016490:1838944-1839306 [Halomonas sp. GFAJ-1]
ATGAGCGTCCCTCAAATTATTCAAACGCCTACCGGAGAGCGGTTGGTGGTATTGCCTGAGCAGGAGTACTACGCCTTGCT
TGCGCGATTAGAAGATCAGGATGATATCGACGCAGCCAATGCGCTAGTGGCGCGCGGTGAAGAGTCGTTTCCGGAAGTGG
TCGTGGATGCGCTACTGGAAGGCGTGTCCCCCGTGAAGGTGTACCGCGAATATCGTGGCCTTCGTGCTGGAGAGCTGGCG
GAAAAAGCAGGCATTAGCCAGGGCTACCTTTCTGAAATAGAGTCAGGCAAAAAGAGTGGCTCATTGAGTGTGCTGAAACG
CATTGCGGCTGCTTTAGATGTGGAATTGAGTGACTTAACCTAG