Detailed information of TA system TA064692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 1710566..1711068
• Replicon: chromosome
• Accession: NZ_CP016490
• Organism: Halomonas sp. GFAJ-1 strain Halomonas sp. GFAJ-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: H0J070
• Locus tag: BB497_RS07880
• Protein ID: WP_009097236.1
• Coordinates: 1710566..1710820 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H0J069
• Locus tag: BB497_RS07885
• Protein ID: WP_009097234.1
• Coordinates: 1710817..1711068 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB497_RS07855	1705790..1706569	+	780	WP_009101849.1	AAA family ATPase	-
BB497_RS07860	1706713..1707566	+	854	WP_105208820.1	IS5 family transposase	-
BB497_RS07865	1707478..1708005	+	528	WP_168183920.1	hypothetical protein	-
BB497_RS07870	1708123..1708302	+	180	WP_009101872.1	hypothetical protein	-
BB497_RS07875	1708416..1710500	+	2085	WP_105208853.1	N-6 DNA methylase	-
BB497_RS07880	1710566..1710820	-	255	WP_009097236.1	Txe/YoeB family addiction module toxin	Toxin
BB497_RS07885	1710817..1711068	-	252	WP_009097234.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BB497_RS07890	1711097..1711600	+	504	WP_202813482.1	hypothetical protein	-
BB497_RS07900	1712314..1712583	+	270	WP_009101227.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BB497_RS07905	1712584..1712883	+	300	WP_009101225.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BB497_RS07910	1712955..1713776	-	822	WP_009101223.1	aldo/keto reductase	-
BB497_RS07915	1714035..1714667	+	633	WP_009101221.1	hypothetical protein	-
BB497_RS07920	1714824..1715708	+	885	WP_009101219.1	class A beta-lactamase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1704609..1712883	8274
	-	inside	Integron	-	-	1707610..1712583	4973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10306.90 Da
• Isoelectric Point: 9.8162

>T64692 WP_009097236.1 NZ_CP016490:c1710820-1710566 [Halomonas sp. GFAJ-1]
MKLIFSENAWEDYLYWQKTDKKIMNRINKLIKETKREPFEGIGKPEPLKHSFAGYWSRRINDEHRMVYKVTDDALLLAQL
RYHY

Nucleotide

• Length: 255 bp

>T64692 NZ_CP016490:c1710820-1710566 [Halomonas sp. GFAJ-1]
ATGAAGCTCATCTTCTCCGAAAACGCCTGGGAAGATTATCTATACTGGCAAAAAACCGACAAAAAGATCATGAATCGAAT
CAATAAACTAATCAAGGAAACGAAACGAGAACCGTTCGAAGGCATCGGCAAACCGGAACCCCTCAAACACAGTTTCGCTG
GCTACTGGTCTCGCCGAATTAACGACGAGCACCGCATGGTCTACAAGGTTACGGATGACGCATTACTCCTGGCCCAGCTC
CGGTACCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9329.51 Da
• Isoelectric Point: 4.4621

>AT64692 WP_009097234.1 NZ_CP016490:c1711068-1710817 [Halomonas sp. GFAJ-1]
MDAISYTAARTNLAKTMEQVCEDHSPVIITRSKSQSVVMISLDDYEALQETSYLLRAPKNARRLLESVTELEQGGGQERE
LLE

Nucleotide

• Length: 252 bp

>AT64692 NZ_CP016490:c1711068-1710817 [Halomonas sp. GFAJ-1]
ATGGATGCCATCAGCTACACAGCCGCTAGAACCAATCTAGCAAAAACTATGGAGCAGGTCTGTGAAGACCATTCTCCAGT
GATCATCACCCGGAGCAAGTCGCAGTCCGTGGTAATGATCTCCCTTGACGATTATGAAGCGTTGCAAGAGACTTCATATC
TCCTGCGCGCACCAAAGAACGCCCGTCGTTTGTTGGAATCCGTTACCGAGTTGGAGCAAGGCGGCGGCCAAGAACGGGAA
CTTCTTGAATGA

Structures

Toxin

• AlphaFold DB: H0J070

Antitoxin

• AlphaFold DB: H0J069