Detailed information of TA system TA064691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 81893..82473
• Replicon: plasmid unnamed6
• Accession: NZ_CP016489
• Organism: Synechococcus sp. PCC 8807

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AWQ24_RS15440
• Protein ID: WP_041444131.1
• Coordinates: 81893..82237 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1B1U2U2
• Locus tag: AWQ24_RS15445
• Protein ID: WP_030008115.1
• Coordinates: 82231..82473 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ24_RS15420	77201..79786	+	2586	WP_065717592.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
AWQ24_RS15425	79912..80277	+	366	WP_041444158.1	VOC family protein	-
AWQ24_RS15430	80342..81229	+	888	WP_143589451.1	ABC transporter substrate-binding protein	-
AWQ24_RS15435	81294..81830	+	537	WP_065717537.1	hypothetical protein	-
AWQ24_RS15440	81893..82237	-	345	WP_041444131.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWQ24_RS15445	82231..82473	-	243	WP_030008115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AWQ24_RS15450	82578..83393	-	816	WP_065717538.1	ABC transporter ATP-binding protein	-
AWQ24_RS15455	83397..84434	-	1038	WP_065717539.1	iron ABC transporter permease	-
AWQ24_RS15460	84434..85405	-	972	WP_065717593.1	iron ABC transporter permease	-
AWQ24_RS15465	85524..86429	-	906	WP_065717540.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..165618	165618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12861.20 Da
• Isoelectric Point: 9.1547

>T64691 WP_041444131.1 NZ_CP016489:c82237-81893 [Synechococcus sp. PCC 8807]
MVKINGMVPKRGDIIFLELNPRTGSEQSGYRPAIVISPEEYNRISNLTLICPITNKEKGWVFEVKLPPNLKTQGVILVDQ
IRAVDCRARKAKIVEQLPLDTLNEVLARLEPLLI

Nucleotide

• Length: 345 bp

>T64691 NZ_CP016489:c82237-81893 [Synechococcus sp. PCC 8807]
TTGGTAAAAATTAACGGCATGGTTCCTAAGCGGGGGGATATTATTTTTCTCGAACTGAATCCCCGCACAGGTAGTGAACA
ATCAGGTTATCGTCCGGCGATCGTGATTTCTCCTGAGGAATATAACCGTATTTCTAATCTGACCCTAATCTGTCCAATCA
CGAATAAAGAAAAAGGCTGGGTGTTTGAGGTGAAACTTCCGCCTAATCTCAAAACCCAAGGCGTCATCCTGGTCGATCAA
ATCCGGGCGGTGGACTGTCGAGCCAGGAAAGCAAAAATTGTGGAACAACTTCCCCTTGACACTCTTAATGAAGTACTCGC
GAGGTTAGAGCCTCTTTTGATTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9119.28 Da
• Isoelectric Point: 4.5125

>AT64691 WP_030008115.1 NZ_CP016489:c82473-82231 [Synechococcus sp. PCC 8807]
MITQKITKWGNSFGIRLPQSMLQQCGWQEGEQVTISMENNQIILSLARPKYSLAELLKDAKPEAQHSEVDWGEAQGEESW

Nucleotide

• Length: 243 bp

>AT64691 NZ_CP016489:c82473-82231 [Synechococcus sp. PCC 8807]
ATGATCACCCAAAAAATCACGAAATGGGGCAACTCTTTTGGTATTCGCTTGCCTCAATCCATGCTCCAGCAATGCGGTTG
GCAGGAAGGGGAACAAGTGACGATATCAATGGAAAACAACCAAATTATTTTGAGCCTTGCTCGACCAAAATATAGCCTTG
CGGAATTACTCAAGGATGCAAAACCTGAAGCACAGCATTCTGAAGTGGACTGGGGGGAAGCCCAAGGAGAAGAGTCTTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1B1U2U2