Detailed information of TA system TA064688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 17396..17976
• Replicon: plasmid unnamed5
• Accession: NZ_CP016482
• Organism: Synechococcus sp. PCC 7117

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AWQ22_RS15160
• Protein ID: WP_157094663.1
• Coordinates: 17396..17722 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1B1U2U2
• Locus tag: AWQ22_RS15165
• Protein ID: WP_030008115.1
• Coordinates: 17734..17976 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ22_RS15140	12703..15288	+	2586	WP_065712016.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
AWQ22_RS15145	15414..15779	+	366	WP_065712017.1	VOC family protein	-
AWQ22_RS15150	15839..16732	+	894	WP_062436973.1	ABC transporter substrate-binding protein	-
AWQ22_RS15155	16797..17333	+	537	WP_065711839.1	hypothetical protein	-
AWQ22_RS15160	17396..17722	-	327	WP_157094663.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AWQ22_RS15165	17734..17976	-	243	WP_030008115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AWQ22_RS16465	18141..18311	+	171	WP_198166563.1	hypothetical protein	-
AWQ22_RS15170	18354..18842	-	489	WP_065711841.1	hypothetical protein	-
AWQ22_RS15175	19106..19921	-	816	WP_065711842.1	ABC transporter ATP-binding protein	-
AWQ22_RS15180	19925..20962	-	1038	WP_065711843.1	iron ABC transporter permease	-
AWQ22_RS15185	20959..21933	-	975	WP_065712018.1	iron ABC transporter permease	-
AWQ22_RS15190	22051..22956	-	906	WP_065711844.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..264782	264782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12177.33 Da
• Isoelectric Point: 8.5043

>T64688 WP_157094663.1 NZ_CP016482:c17722-17396 [Synechococcus sp. PCC 7117]
MVPKRGDIIFLELNPRTGSEQSGYRPAIVISPEEYNRISNLTLICPITSKEKGWVFEVKLPPNIKTQGVILVDQIRAVDC
RARKAKIVEQLPLDTLNDVLARLEPLLI

Nucleotide

• Length: 327 bp

>T64688 NZ_CP016482:c17722-17396 [Synechococcus sp. PCC 7117]
ATGGTTCCTAAGCGGGGGGATATTATTTTTCTCGAACTGAATCCCCGCACAGGTAGTGAACAATCAGGTTATCGTCCGGC
GATCGTGATTTCTCCTGAGGAATATAACCGTATTTCTAATCTGACCCTAATCTGTCCAATCACGAGTAAAGAAAAAGGCT
GGGTGTTTGAGGTGAAACTTCCGCCTAATATCAAAACCCAAGGCGTCATCCTGGTCGATCAAATCCGGGCGGTGGACTGT
CGAGCCAGGAAAGCAAAAATTGTGGAACAACTTCCCCTTGACACTCTTAATGACGTACTCGCGAGGTTAGAGCCTCTTTT
GATTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9119.28 Da
• Isoelectric Point: 4.5125

>AT64688 WP_030008115.1 NZ_CP016482:c17976-17734 [Synechococcus sp. PCC 7117]
MITQKITKWGNSFGIRLPQSMLQQCGWQEGEQVTISMENNQIILSLARPKYSLAELLKDAKPEAQHSEVDWGEAQGEESW

Nucleotide

• Length: 243 bp

>AT64688 NZ_CP016482:c17976-17734 [Synechococcus sp. PCC 7117]
ATGATCACCCAAAAAATCACGAAATGGGGCAACTCTTTTGGTATTCGTTTGCCTCAATCCATGCTCCAGCAATGCGGTTG
GCAGGAAGGGGAACAGGTGACGATATCAATGGAAAACAACCAAATTATTTTGAGCCTTGCTCGACCAAAATATAGCCTTG
CGGAATTACTCAAGGATGCAAAACCTGAAGCACAGCATTCTGAAGTGGATTGGGGGGAAGCCCAAGGAGAAGAGTCTTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1B1U2U2