Detailed information of TA system TA064681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1772524..1773122
• Replicon: chromosome
• Accession: NZ_CP016474
• Organism: Synechococcus sp. PCC 7003

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AWQ21_RS15910
• Protein ID: WP_071932277.1
• Coordinates: 1772895..1773122 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1B1TPW1
• Locus tag: AWQ21_RS08435
• Protein ID: WP_065714158.1
• Coordinates: 1772524..1772871 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ21_RS08400	1767990..1768274	+	285	WP_065714151.1	DUF2470 domain-containing protein	-
AWQ21_RS08405	1768502..1768783	+	282	WP_065714152.1	hypothetical protein	-
AWQ21_RS08410	1768920..1769789	+	870	WP_065714153.1	M48 family metalloprotease	-
AWQ21_RS08415	1769929..1770414	-	486	WP_065714154.1	DUF4168 domain-containing protein	-
AWQ21_RS08420	1770535..1771515	-	981	WP_065714155.1	DNA polymerase III subunit delta	-
AWQ21_RS08425	1771584..1772105	+	522	WP_065714156.1	photosystem I assembly protein Ycf3	-
AWQ21_RS08430	1772161..1772454	+	294	WP_065714157.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
AWQ21_RS08435	1772524..1772871	-	348	WP_065714158.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AWQ21_RS15910	1772895..1773122	-	228	WP_071932277.1	type II toxin-antitoxin system HicA family toxin	Toxin
AWQ21_RS08440	1773344..1773745	+	402	WP_065714159.1	preQ(1) synthase	-
AWQ21_RS08445	1773742..1774617	+	876	WP_198159629.1	prolipoprotein diacylglyceryl transferase	-
AWQ21_RS08450	1774654..1775211	+	558	WP_065714160.1	bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase	-
AWQ21_RS08455	1775526..1776584	+	1059	WP_012307174.1	photosystem II D2 protein (photosystem q(a) protein)	-
AWQ21_RS08460	1776568..1777950	+	1383	WP_065714161.1	photosystem II reaction center protein CP43	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8281.66 Da
• Isoelectric Point: 11.3076

>T64681 WP_071932277.1 NZ_CP016474:c1773122-1772895 [Synechococcus sp. PCC 7003]
MKRKNSKTLALIFQRPISGNIAWSDIEGLLLELGAEISERSGSRVAVILFGEVRVFHRPHPSPKTDKGAVASIRK

Nucleotide

• Length: 228 bp

>T64681 NZ_CP016474:c1773122-1772895 [Synechococcus sp. PCC 7003]
ATGAAGCGTAAAAACAGTAAAACCTTAGCGCTTATTTTTCAAAGGCCCATCAGTGGCAACATTGCCTGGTCTGACATTGA
GGGGCTCTTGCTGGAACTAGGAGCAGAAATTTCCGAGAGAAGCGGATCGCGAGTTGCTGTAATTCTCTTTGGTGAAGTGC
GGGTTTTTCATCGACCTCACCCTTCCCCTAAAACTGATAAAGGAGCCGTTGCCAGCATTCGGAAATAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13184.00 Da
• Isoelectric Point: 4.5942

>AT64681 WP_065714158.1 NZ_CP016474:c1772871-1772524 [Synechococcus sp. PCC 7003]
MNMMILDGYRAKIEYDPELDLFRGEILGLNGGADFYGSNPAELRQEFRNSLNTFLEVCAEKGIKPTKDYSGRFNLRVPPE
LHREISMITTSKNISINKWITEILQEAVQSVDQEI

Nucleotide

• Length: 348 bp

>AT64681 NZ_CP016474:c1772871-1772524 [Synechococcus sp. PCC 7003]
ATGAACATGATGATTCTTGATGGTTATCGAGCAAAAATTGAGTATGATCCCGAACTCGACTTATTTCGCGGCGAAATCTT
GGGGTTAAATGGCGGCGCAGATTTTTATGGTAGTAATCCAGCAGAACTCCGCCAGGAATTTAGAAATTCTCTTAATACTT
TTCTCGAAGTCTGTGCCGAAAAAGGGATTAAGCCAACAAAAGACTATTCAGGACGGTTTAATTTGCGAGTGCCGCCGGAG
CTACACCGGGAAATCTCAATGATTACGACCAGCAAAAATATTAGCATTAACAAGTGGATTACAGAGATTCTGCAGGAAGC
GGTGCAGTCTGTTGATCAAGAAATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1B1TPW1