Detailed information of TA system TA064678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 876799..877382
• Replicon: chromosome
• Accession: NZ_CP016474
• Organism: Synechococcus sp. PCC 7003

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AWQ21_RS04175
• Protein ID: WP_198159711.1
• Coordinates: 877038..877382 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1B1TMQ6
• Locus tag: AWQ21_RS04170
• Protein ID: WP_065713462.1
• Coordinates: 876799..877041 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWQ21_RS04155	872732..873208	+	477	WP_065713459.1	Hsp20/alpha crystallin family protein	-
AWQ21_RS04160	873483..874853	+	1371	WP_157094693.1	hypothetical protein	-
AWQ21_RS04165	874947..876632	+	1686	WP_065713461.1	dihydroxy-acid dehydratase	-
AWQ21_RS04170	876799..877041	+	243	WP_065713462.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AWQ21_RS04175	877038..877382	+	345	WP_198159711.1	endoribonuclease MazF	Toxin
AWQ21_RS04180	877424..879121	+	1698	WP_065713464.1	DNA mismatch repair endonuclease MutL	-
AWQ21_RS04185	879166..880701	+	1536	WP_065713465.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
AWQ21_RS04190	880723..881112	-	390	WP_065713466.1	DUF2358 domain-containing protein	-
AWQ21_RS04195	881128..881952	-	825	WP_157094694.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13095.29 Da
• Isoelectric Point: 10.1387

>T64678 WP_198159711.1 NZ_CP016474:877038-877382 [Synechococcus sp. PCC 7003]
VKHHQVYIPRRGDLIFLDFNPTKGHEQSGHRPAFVISPQTYNAKTSLALLIPITKQRKGYPFEVPLSPNLQIQGVILVDQ
IKCLDWQVRQVRFVETAPQNIVEEVQAKLKPLIF

Nucleotide

• Length: 345 bp

>T64678 NZ_CP016474:877038-877382 [Synechococcus sp. PCC 7003]
GTGAAACATCATCAAGTTTATATTCCCCGTCGCGGAGATCTTATTTTTCTAGATTTCAACCCGACTAAAGGCCATGAGCA
AAGTGGCCACCGACCTGCTTTCGTCATCTCGCCCCAAACCTATAACGCCAAAACTTCGCTGGCATTGCTGATCCCGATCA
CAAAACAGCGGAAAGGCTATCCTTTTGAAGTGCCACTGTCGCCCAATTTACAAATCCAAGGTGTGATTTTAGTTGATCAA
ATCAAATGTTTAGATTGGCAAGTCCGGCAAGTCCGGTTTGTGGAAACAGCTCCCCAAAATATTGTTGAAGAAGTTCAAGC
AAAGCTAAAGCCATTAATTTTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8688.89 Da
• Isoelectric Point: 4.2259

>AT64678 WP_065713462.1 NZ_CP016474:876799-877041 [Synechococcus sp. PCC 7003]
MITTTAKWGNSLAIRIPKNLAEELALTEKSTVDLRVIEGKIVITPQTAPSYSLEELLEAITPDNLHGEIDSGLATGNEVW

Nucleotide

• Length: 243 bp

>AT64678 NZ_CP016474:876799-877041 [Synechococcus sp. PCC 7003]
ATGATCACAACAACCGCAAAGTGGGGCAATAGCCTTGCCATCCGGATTCCTAAAAATTTAGCCGAGGAGCTTGCTCTCAC
TGAAAAATCAACTGTCGATTTAAGGGTGATTGAAGGAAAAATTGTGATCACGCCACAAACCGCTCCCAGCTATTCCCTAG
AGGAACTTCTAGAAGCGATTACACCGGACAATCTCCACGGAGAAATTGACAGCGGCCTCGCCACAGGAAATGAAGTGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1B1TMQ6