Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3402221..3402801 Replicon chromosome
Accession NZ_CP016464
Organism Bosea sp. RAC05

Toxin (Protein)


Gene name pemK Uniprot ID A0A1B3NE55
Locus tag BSY19_RS19580 Protein ID WP_069055596.1
Coordinates 3402475..3402801 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1B3NLR4
Locus tag BSY19_RS19575 Protein ID WP_069055595.1
Coordinates 3402221..3402478 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY19_RS19555 3399143..3399583 + 441 WP_069055591.1 hypothetical protein -
BSY19_RS19560 3399714..3400697 + 984 WP_069055592.1 MBL fold metallo-hydrolase -
BSY19_RS19565 3400848..3401621 + 774 WP_069055593.1 NAD kinase -
BSY19_RS19570 3401708..3402130 + 423 WP_069055594.1 carboxymuconolactone decarboxylase family protein -
BSY19_RS19575 3402221..3402478 + 258 WP_069055595.1 antitoxin Antitoxin
BSY19_RS19580 3402475..3402801 + 327 WP_069055596.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BSY19_RS19585 3402850..3404064 - 1215 WP_069055597.1 DUF2336 domain-containing protein -
BSY19_RS19590 3404061..3405143 - 1083 WP_069055598.1 hypothetical protein -
BSY19_RS19595 3405340..3405837 + 498 WP_066725808.1 MaoC family dehydratase -
BSY19_RS19600 3405843..3406721 + 879 WP_069055599.1 CoA ester lyase -
BSY19_RS19605 3406741..3406947 + 207 WP_069055600.1 hypothetical protein -
BSY19_RS19610 3406991..3407596 - 606 WP_069055601.1 flavin reductase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11916.67 Da        Isoelectric Point: 7.1677

>T64673 WP_069055596.1 NZ_CP016464:3402475-3402801 [Bosea sp. RAC05]
VKRGDVYMVDLEPTHGREQRGHRPVVIVSPDAFNQATQLPVVAPITNGGDFARRIGFAVPLEGTSTSGVIRCDQPRVLDL
KARNGRRIETLPPEIMDEVLARLATLFE

Download         Length: 327 bp

>T64673 NZ_CP016464:3402475-3402801 [Bosea sp. RAC05]
GTGAAGCGCGGCGATGTCTACATGGTCGACCTCGAGCCGACCCACGGACGCGAACAGCGCGGGCATCGGCCGGTCGTCAT
CGTCTCGCCGGATGCGTTCAATCAGGCGACCCAGTTGCCCGTCGTCGCGCCGATCACGAATGGCGGCGACTTCGCGCGTC
GCATCGGCTTTGCGGTCCCGTTGGAGGGAACCAGTACCAGCGGCGTGATCCGCTGTGACCAGCCCCGTGTTCTCGACCTG
AAAGCTCGTAACGGCCGCAGGATCGAGACCCTCCCACCGGAGATCATGGACGAGGTGCTGGCGCGTCTCGCAACGCTCTT
CGAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9098.43 Da        Isoelectric Point: 4.9861

>AT64673 WP_069055595.1 NZ_CP016464:3402221-3402478 [Bosea sp. RAC05]
MHTTNLRKVGGSIMLAVPPALLDVLRLSAGAKVGLAVDNGRLVVEPQARPRYTMAELLAASDYSQQQPSSEREWVDAPAA
GEELL

Download         Length: 258 bp

>AT64673 NZ_CP016464:3402221-3402478 [Bosea sp. RAC05]
ATGCACACGACCAATCTGCGCAAGGTCGGCGGCTCGATCATGCTCGCCGTCCCGCCGGCCCTGCTCGACGTCTTGCGCCT
CTCCGCGGGCGCCAAGGTCGGACTGGCCGTCGACAATGGCCGCCTGGTCGTCGAGCCGCAGGCCAGACCCCGTTACACCA
TGGCCGAACTGCTCGCGGCGTCTGATTATTCCCAACAGCAGCCGTCCAGCGAGCGCGAATGGGTCGATGCGCCGGCCGCC
GGTGAAGAGCTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NE55

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NLR4

References