Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 2331154..2331698 Replicon chromosome
Accession NZ_CP016464
Organism Bosea sp. RAC05

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B3NRD9
Locus tag BSY19_RS14495 Protein ID WP_069054780.1
Coordinates 2331154..2331477 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B3NKU5
Locus tag BSY19_RS14500 Protein ID WP_069054781.1
Coordinates 2331474..2331698 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY19_RS14475 2326833..2327888 + 1056 WP_069054777.1 enoyl-CoA hydratase/isomerase family protein -
BSY19_RS14480 2327893..2328342 + 450 WP_069054778.1 hypothetical protein -
BSY19_RS14485 2328546..2329058 + 513 WP_069054779.1 winged helix DNA-binding protein -
BSY19_RS14490 2329333..2330565 + 1233 WP_069057115.1 L,D-transpeptidase family protein -
BSY19_RS14495 2331154..2331477 - 324 WP_069054780.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BSY19_RS14500 2331474..2331698 - 225 WP_069054781.1 antitoxin MazE family protein Antitoxin
BSY19_RS14505 2331773..2332963 - 1191 WP_069057116.1 Na+/H+ antiporter NhaA -
BSY19_RS14510 2333173..2334474 + 1302 WP_069057117.1 serine hydroxymethyltransferase -
BSY19_RS14515 2334531..2334764 + 234 WP_150129626.1 hypothetical protein -
BSY19_RS14520 2334766..2335209 + 444 WP_171905145.1 hypothetical protein -
BSY19_RS14525 2335229..2335726 + 498 WP_069054784.1 transcriptional regulator NrdR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(6-70)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11383.37 Da        Isoelectric Point: 10.4581

>T64671 WP_069054780.1 NZ_CP016464:c2331477-2331154 [Bosea sp. RAC05]
VTRGEIVTVAMTGDFGKPRPALVVHADPFDQTGTITVLLISGTLVDAPLIRLTVQPTPQNGLLKPSQIQIDKAMSVQRHR
VGAVIGRLDDTTMLAVTRSLALFLGIA

Download         Length: 324 bp

>T64671 NZ_CP016464:c2331477-2331154 [Bosea sp. RAC05]
GTGACGCGTGGCGAGATCGTCACCGTCGCGATGACGGGCGATTTCGGAAAGCCCCGCCCTGCCCTCGTCGTCCATGCCGA
TCCTTTCGATCAGACCGGCACCATCACCGTTCTGCTGATCTCGGGAACGCTGGTCGATGCCCCCCTGATCCGCCTGACAG
TCCAGCCGACCCCACAGAACGGACTGCTCAAACCCTCGCAGATCCAGATCGACAAGGCGATGTCGGTCCAGCGGCACAGG
GTCGGCGCCGTCATAGGAAGACTGGACGATACGACCATGCTGGCGGTGACGCGGTCACTGGCGCTGTTTCTCGGGATTGC
GTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8159.17 Da        Isoelectric Point: 4.7085

>AT64671 WP_069054781.1 NZ_CP016464:c2331698-2331474 [Bosea sp. RAC05]
MPTPVNQRVQKRRDALRAAGLRPVQIWVPDTRRPGFVEECRRQSLIVAAADADDNGLNSFLEAALADLTGEDEA

Download         Length: 225 bp

>AT64671 NZ_CP016464:c2331698-2331474 [Bosea sp. RAC05]
ATGCCCACACCCGTCAACCAGCGGGTTCAGAAGCGTCGCGATGCGCTGCGGGCCGCCGGGCTGCGGCCCGTTCAGATTTG
GGTTCCAGACACCCGCCGTCCGGGTTTTGTCGAAGAGTGCCGCCGTCAGTCGCTCATTGTCGCAGCGGCGGACGCCGACG
ATAACGGCCTGAACAGCTTTCTCGAGGCCGCTCTGGCCGATCTCACCGGCGAGGACGAGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NRD9

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NKU5

References