Detailed information of TA system TA064670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2264869..2265541
• Replicon: chromosome
• Accession: NZ_CP016464
• Organism: Bosea sp. RAC05

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BSY19_RS14195
• Protein ID: WP_069054727.1
• Coordinates: 2265359..2265541 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1B3NLU3
• Locus tag: BSY19_RS14190
• Protein ID: WP_069054726.1
• Coordinates: 2264869..2265321 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSY19_RS14165	2259942..2260682	-	741	WP_069054722.1	metallophosphatase family protein	-
BSY19_RS14170	2260789..2262027	-	1239	WP_069054723.1	aspartate kinase	-
BSY19_RS14175	2262178..2262948	+	771	WP_069054724.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
BSY19_RS14180	2262973..2263308	-	336	WP_069054725.1	hypothetical protein	-
BSY19_RS14185	2263574..2264845	+	1272	WP_066720525.1	crotonyl-CoA carboxylase/reductase	-
BSY19_RS14190	2264869..2265321	-	453	WP_069054726.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BSY19_RS14195	2265359..2265541	-	183	WP_069054727.1	type II toxin-antitoxin system HicA family toxin	Toxin
BSY19_RS14200	2265748..2266407	+	660	WP_069054728.1	glutathione S-transferase family protein	-
BSY19_RS14205	2266515..2267396	+	882	WP_083247917.1	DMT family transporter	-
BSY19_RS14210	2267518..2269224	+	1707	WP_069054730.1	acyl-CoA dehydrogenase family protein	-
BSY19_RS14215	2269307..2270377	+	1071	WP_069054731.1	HD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (16-141)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6813.87 Da
• Isoelectric Point: 11.1367

>T64670 WP_069054727.1 NZ_CP016464:c2265541-2265359 [Bosea sp. RAC05]
MDSRDLIKELERDGWVRHSVTGSHHHFKHPSKPGKVTLPPPVKDMHPKTVKSVRRQAGLA

Nucleotide

• Length: 183 bp

>T64670 NZ_CP016464:c2265541-2265359 [Bosea sp. RAC05]
ATGGATTCGCGCGACTTGATCAAGGAACTGGAGCGCGATGGATGGGTGCGTCACTCCGTGACCGGCAGCCATCACCATTT
CAAACACCCCTCGAAGCCCGGCAAGGTCACGCTGCCGCCCCCCGTCAAGGACATGCATCCAAAAACGGTGAAATCCGTGA
GACGGCAGGCGGGTCTGGCTTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16120.34 Da
• Isoelectric Point: 5.6620

>AT64670 WP_069054726.1 NZ_CP016464:c2265321-2264869 [Bosea sp. RAC05]
MSASYFALLHLPEPGSATWGVTFPDLPGCVSSGATDEEARKKAREALAGHIAALRADGDDVPHARTLKELLGDPDIISEI
KDIFVDEHLRSGAVLENIPLDDIAPPKERINVMLDRKVLRAIDEAARARGVSRSAFIEQTAGKAALKRSA

Nucleotide

• Length: 453 bp

>AT64670 NZ_CP016464:c2265321-2264869 [Bosea sp. RAC05]
ATGTCGGCCAGCTATTTTGCCCTGCTTCATTTGCCCGAGCCGGGATCGGCGACCTGGGGTGTGACCTTTCCCGATCTGCC
CGGCTGCGTTTCGTCGGGAGCCACGGATGAGGAAGCCAGAAAGAAGGCGCGAGAAGCCCTTGCCGGCCATATCGCAGCGT
TGCGCGCGGACGGGGACGACGTTCCCCACGCCCGGACCCTGAAAGAACTTCTCGGCGATCCTGACATCATCTCGGAGATC
AAGGACATCTTCGTCGATGAGCATCTGCGGAGCGGAGCGGTTCTGGAGAACATTCCGCTCGACGATATCGCCCCGCCGAA
GGAGCGCATCAACGTCATGCTGGACCGGAAGGTCCTGAGGGCCATCGACGAGGCGGCGCGCGCCCGCGGCGTGAGCCGGT
CGGCCTTCATCGAGCAGACGGCGGGCAAGGCGGCGCTGAAGCGCTCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B3NLU3