Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 2014056..2014527 Replicon chromosome
Accession NZ_CP016464
Organism Bosea sp. RAC05

Toxin (Protein)


Gene name - Uniprot ID A0A1B3NJT5
Locus tag BSY19_RS12970 Protein ID WP_069054534.1
Coordinates 2014056..2014319 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1B3NJU0
Locus tag BSY19_RS12975 Protein ID WP_069054535.1
Coordinates 2014306..2014527 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY19_RS12945 2010144..2010977 + 834 WP_069054531.1 sulfite exporter TauE/SafE family protein -
BSY19_RS12950 2011075..2011485 + 411 WP_083247911.1 GNAT family N-acetyltransferase -
BSY19_RS12955 2011514..2011756 - 243 WP_083247576.1 hypothetical protein -
BSY19_RS27015 2011821..2012000 - 180 WP_083247912.1 DUF1289 domain-containing protein -
BSY19_RS12960 2012006..2012884 - 879 WP_069054532.1 adenosylcobinamide-GDP ribazoletransferase -
BSY19_RS12965 2012966..2013985 + 1020 WP_069054533.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -
BSY19_RS12970 2014056..2014319 + 264 WP_069054534.1 BrnT family toxin Toxin
BSY19_RS12975 2014306..2014527 + 222 WP_069054535.1 CopG family transcriptional regulator Antitoxin
BSY19_RS12980 2014595..2015716 - 1122 WP_083247577.1 D-alanine--D-alanine ligase -
BSY19_RS12985 2015811..2017328 - 1518 WP_069054537.1 HAMP domain-containing histidine kinase -
BSY19_RS12990 2017415..2017966 + 552 WP_150129603.1 thermonuclease family protein -
BSY19_RS12995 2018070..2018681 + 612 WP_069057065.1 glutathione S-transferase family protein -
BSY19_RS13000 2018681..2019151 + 471 WP_069054538.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-80)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10038.36 Da        Isoelectric Point: 6.4688

>T64669 WP_069054534.1 NZ_CP016464:2014056-2014319 [Bosea sp. RAC05]
MPFEFDPLKSAANLAKHGIDFQQAQALWEDARLLEAPARTEGEPRFMAVGLIGQKHWSAIYVYRGDRVRIISVRRARSEE
IEYYENG

Download         Length: 264 bp

>T64669 NZ_CP016464:2014056-2014319 [Bosea sp. RAC05]
ATGCCATTTGAGTTCGACCCTCTCAAAAGCGCGGCCAACTTGGCCAAGCACGGGATCGATTTCCAACAGGCGCAGGCTCT
TTGGGAGGATGCGCGTCTGCTGGAAGCGCCTGCCCGGACTGAGGGCGAGCCGCGCTTCATGGCAGTGGGACTGATCGGCC
AAAAGCACTGGTCCGCGATTTATGTCTATCGCGGCGATCGGGTGCGTATCATCTCGGTGCGGCGCGCTCGAAGTGAGGAG
ATCGAATACTATGAAAACGGGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8355.40 Da        Isoelectric Point: 8.7695

>AT64669 WP_069054535.1 NZ_CP016464:2014306-2014527 [Bosea sp. RAC05]
MKTGDFDQAFDDGQSVEDKIDWAKARRPNSETKRVNVDFPSWVVQGLDRQAQHLGVTRQSLIKLWIAERLGKA

Download         Length: 222 bp

>AT64669 NZ_CP016464:2014306-2014527 [Bosea sp. RAC05]
ATGAAAACGGGTGATTTCGATCAGGCTTTCGATGACGGGCAGTCTGTTGAGGACAAAATCGACTGGGCCAAGGCGCGGCG
TCCCAACAGCGAGACGAAGCGCGTGAACGTCGATTTTCCGAGTTGGGTCGTCCAAGGCCTGGACCGTCAGGCGCAGCATC
TCGGCGTGACCCGGCAGTCCCTGATCAAGCTCTGGATTGCGGAGCGACTGGGCAAGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NJT5

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NJU0

References