Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-DUF971
Location 1818512..1819112 Replicon chromosome
Accession NZ_CP016464
Organism Bosea sp. RAC05

Toxin (Protein)


Gene name relE Uniprot ID A0A1B3NE65
Locus tag BSY19_RS12055 Protein ID WP_069054383.1
Coordinates 1818804..1819112 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1B3NRL0
Locus tag BSY19_RS12050 Protein ID WP_069054382.1
Coordinates 1818512..1818820 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY19_RS12035 1814136..1815776 + 1641 WP_069054379.1 ABC transporter substrate-binding protein -
BSY19_RS12040 1815782..1817194 + 1413 WP_069054380.1 amidase -
BSY19_RS12045 1817275..1818498 + 1224 WP_069054381.1 MFS transporter -
BSY19_RS12050 1818512..1818820 - 309 WP_069054382.1 putative addiction module antidote protein Antitoxin
BSY19_RS12055 1818804..1819112 - 309 WP_069054383.1 hypothetical protein Toxin
BSY19_RS12060 1819178..1820098 - 921 WP_069054384.1 pirin family protein -
BSY19_RS12065 1820314..1821174 + 861 WP_069054385.1 N-acetylmuramoyl-L-alanine amidase -
BSY19_RS12070 1821202..1821459 - 258 WP_069054386.1 exodeoxyribonuclease VII small subunit -
BSY19_RS12075 1821552..1821992 - 441 WP_069054387.1 hypothetical protein -
BSY19_RS12080 1822016..1822474 - 459 WP_211201369.1 Lrp/AsnC ligand binding domain-containing protein -
BSY19_RS12085 1822630..1823142 - 513 WP_069054389.1 protein tyrosine phosphatase -
BSY19_RS12090 1823346..1823909 + 564 WP_069054390.1 thioesterase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(7-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(51-94)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11656.36 Da        Isoelectric Point: 10.3735

>T64668 WP_069054383.1 NZ_CP016464:c1819112-1818804 [Bosea sp. RAC05]
MMNFEVLPAFDRWLAEIRDGEAKRRIATRLSRLRLGNPGDGRFLSDGVSELKIDYGPGYRIYYARRGGILILLLGGGDKK
SQSRDIARAVYDLKEWDHARKS

Download         Length: 309 bp

>T64668 NZ_CP016464:c1819112-1818804 [Bosea sp. RAC05]
ATGATGAATTTCGAGGTGCTGCCCGCGTTTGATCGTTGGCTCGCCGAAATTCGCGACGGAGAGGCCAAGCGCCGCATCGC
GACACGTTTGTCGCGGCTCAGACTGGGTAATCCGGGCGATGGCCGCTTTCTGAGCGATGGGGTCAGTGAGCTCAAGATCG
ACTACGGACCCGGCTATCGCATCTACTATGCGAGGCGTGGCGGCATCCTGATTTTGCTCCTTGGTGGAGGCGACAAGAAA
AGTCAGTCCAGGGATATCGCCAGAGCCGTCTACGATCTGAAGGAATGGGATCATGCCCGCAAATCCTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 10686.17 Da        Isoelectric Point: 4.5535

>AT64668 WP_069054382.1 NZ_CP016464:c1818820-1818512 [Bosea sp. RAC05]
MPANPDHISRPFDIAELVTDPDVAQGFLEDAFASGDANEIAEAIGIVARAQGMTTLASEAGMSRETLYRTLSAKGNPTLS
TLLAVLKALGFKLSGVERVKPA

Download         Length: 309 bp

>AT64668 NZ_CP016464:c1818820-1818512 [Bosea sp. RAC05]
ATGCCCGCAAATCCTGACCACATCTCCCGCCCGTTCGACATTGCCGAACTCGTCACCGATCCGGACGTCGCACAGGGATT
TCTCGAAGACGCTTTCGCCTCGGGTGATGCGAACGAGATCGCAGAGGCCATCGGCATCGTCGCCCGCGCCCAAGGCATGA
CCACGCTTGCCAGCGAAGCTGGAATGTCGCGCGAGACGCTCTATCGAACCCTGAGCGCCAAGGGCAATCCGACTCTGAGC
ACGCTTCTGGCCGTTCTGAAAGCGCTCGGCTTCAAGCTCAGCGGCGTCGAGCGGGTCAAGCCTGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NE65

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NRL0

References