Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 599924..600696 Replicon chromosome
Accession NZ_CP016464
Organism Bosea sp. RAC05

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1B3NEK6
Locus tag BSY19_RS06340 Protein ID WP_069053408.1
Coordinates 599924..600412 (-) Length 163 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A1B3NFW6
Locus tag BSY19_RS06345 Protein ID WP_069053409.1
Coordinates 600409..600696 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY19_RS27450 595668..596846 + 1179 WP_069053406.1 fumarylacetoacetate hydrolase family protein -
BSY19_RS27455 596843..597772 + 930 WP_171905095.1 carbohydrate kinase -
BSY19_RS06330 597784..598803 + 1020 WP_069053407.1 proline racemase family protein -
BSY19_RS06335 598840..599736 - 897 WP_069056898.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
BSY19_RS06340 599924..600412 - 489 WP_069053408.1 GNAT family N-acetyltransferase Toxin
BSY19_RS06345 600409..600696 - 288 WP_069053409.1 DUF1778 domain-containing protein Antitoxin
BSY19_RS06350 600755..601300 - 546 WP_069053410.1 DUF2244 domain-containing protein -
BSY19_RS06355 601321..602022 + 702 WP_069053411.1 endonuclease III -
BSY19_RS06360 602031..602672 - 642 WP_150129517.1 hypothetical protein -
BSY19_RS06365 602919..603773 + 855 WP_069056899.1 SDR family NAD(P)-dependent oxidoreductase -
BSY19_RS06370 603767..604711 - 945 WP_069053413.1 glyoxylate/hydroxypyruvate reductase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(73-143)

Antitoxin

Vibrio phage ICP1, Orf50

(1-94)


Sequences

Toxin        


Download         Length: 163 a.a.        Molecular weight: 17173.04 Da        Isoelectric Point: 9.7133

>T64664 WP_069053408.1 NZ_CP016464:c600412-599924 [Bosea sp. RAC05]
VISAPEPLSDSHDLTGFRCGVPTLDDWLRQRALKNQMTGASRTFVACKGTRALAYYALASGAVTATMATGRLRRNMPDPI
PVVVLGRLAIDIGAQGQGLGRALIRDAGLRVLHAADLIGIRGLLVHALSEDAKAFYQRVGFEPSPLAPMTLMITLPDLGA
AI

Download         Length: 489 bp

>T64664 NZ_CP016464:c600412-599924 [Bosea sp. RAC05]
GTGATCTCGGCGCCAGAGCCGCTCTCAGACAGCCATGATCTCACCGGCTTCCGCTGCGGCGTCCCGACACTCGATGACTG
GCTGCGGCAACGCGCGCTCAAAAATCAGATGACCGGCGCATCGCGGACCTTTGTCGCCTGCAAGGGAACCCGCGCCCTCG
CCTATTATGCGCTGGCGTCGGGCGCCGTCACGGCGACGATGGCGACCGGTCGCCTTCGCCGCAACATGCCCGACCCCATA
CCCGTCGTCGTCCTCGGCCGTCTCGCCATCGACATCGGTGCTCAAGGACAAGGGTTGGGGCGGGCCCTGATACGCGATGC
CGGCCTGCGGGTCCTGCATGCTGCCGATTTGATTGGTATTCGAGGGCTCCTCGTGCATGCGCTGTCGGAAGACGCCAAAG
CCTTCTACCAGCGTGTCGGGTTCGAGCCGTCGCCACTGGCTCCGATGACGTTGATGATCACGCTGCCCGATCTCGGCGCG
GCGATTTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10629.03 Da        Isoelectric Point: 5.1486

>AT64664 WP_069053409.1 NZ_CP016464:c600696-600409 [Bosea sp. RAC05]
MTAATASPKRETLNIRIKPEERGLIDRAAQSQGKNRTDFILDAARLAAEEALLSQVVLTVSPEAYAQFLARLDQQPDPNE
RLRKTMQTPAPWDEP

Download         Length: 288 bp

>AT64664 NZ_CP016464:c600696-600409 [Bosea sp. RAC05]
ATGACGGCTGCGACGGCATCACCGAAGCGCGAGACGCTCAACATTCGCATCAAGCCCGAGGAGCGCGGCCTGATCGACCG
CGCAGCCCAAAGCCAGGGCAAGAATCGGACGGACTTCATTCTGGACGCCGCACGACTGGCCGCCGAAGAGGCCCTGCTCA
GTCAGGTCGTCCTTACCGTCAGCCCCGAAGCATACGCACAGTTCCTGGCCAGGCTCGACCAGCAGCCCGATCCGAACGAA
CGGCTGCGCAAGACGATGCAAACCCCTGCCCCCTGGGACGAGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NEK6

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NFW6

References