Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2258673..2259247 Replicon chromosome
Accession NZ_CP016460
Organism Blastomonas sp. RAC04

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B3NB02
Locus tag BSY18_RS11510 Protein ID WP_069050686.1
Coordinates 2258915..2259247 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B3NA96
Locus tag BSY18_RS11505 Protein ID WP_069051881.1
Coordinates 2258673..2258915 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY18_RS11490 2254091..2256580 - 2490 WP_171900221.1 TonB-dependent receptor -
BSY18_RS11495 2256971..2258020 + 1050 WP_069050684.1 CoA transferase -
BSY18_RS11500 2257983..2258552 - 570 WP_150126267.1 hypothetical protein -
BSY18_RS11505 2258673..2258915 + 243 WP_069051881.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BSY18_RS11510 2258915..2259247 + 333 WP_069050686.1 endoribonuclease MazF Toxin
BSY18_RS11515 2259254..2260177 - 924 WP_069050687.1 glutaminase -
BSY18_RS11520 2260277..2261248 + 972 WP_069050688.1 bile acid:sodium symporter -
BSY18_RS11525 2261295..2262257 + 963 WP_054134058.1 radical SAM protein -
BSY18_RS11530 2262424..2263668 + 1245 WP_083231642.1 AarF/ABC1/UbiB kinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

SpoVT-AbrB domain

(6-46)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11839.74 Da        Isoelectric Point: 9.9023

>T64661 WP_069050686.1 NZ_CP016460:2258915-2259247 [Blastomonas sp. RAC04]
VATYVPDAGDIVWLQFDPQAGHEQAGHRPALVLSPASYNEKRGMMICCPMTSRSKGYAFEVAVGDATGSVVLADQIKSVD
WRARKATRKGKAPVATLLDVRAKLKALLSL

Download         Length: 333 bp

>T64661 NZ_CP016460:2258915-2259247 [Blastomonas sp. RAC04]
GTGGCTACCTATGTCCCGGACGCCGGTGACATCGTCTGGCTTCAGTTCGATCCGCAAGCAGGACATGAACAGGCGGGACA
CCGACCAGCTCTGGTCCTTTCGCCCGCGAGCTACAATGAAAAGCGCGGAATGATGATCTGCTGCCCGATGACATCGCGCA
GCAAGGGCTATGCTTTTGAAGTGGCTGTCGGCGATGCCACTGGCAGCGTGGTTCTGGCGGACCAGATCAAGAGTGTCGAC
TGGCGGGCACGCAAGGCCACCCGCAAGGGGAAAGCACCGGTCGCGACCCTTCTGGACGTCCGCGCCAAGCTGAAGGCGCT
GCTCAGCCTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8860.06 Da        Isoelectric Point: 4.0474

>AT64661 WP_069051881.1 NZ_CP016460:2258673-2258915 [Blastomonas sp. RAC04]
MRSQVKKWGNSASVRIPARVLAAASMQINQEVEMREEGGRIIIEPVTVPTYDLDDLLAAMAPETFPDDIDFGSPVGEEIW

Download         Length: 243 bp

>AT64661 NZ_CP016460:2258673-2258915 [Blastomonas sp. RAC04]
ATGCGGAGCCAAGTCAAAAAATGGGGAAACAGCGCGTCTGTTAGAATACCCGCAAGGGTACTTGCAGCGGCTTCCATGCA
GATCAATCAGGAGGTCGAAATGCGTGAGGAGGGCGGGCGGATCATCATCGAGCCGGTGACGGTCCCCACTTACGATCTGG
ATGATCTGCTTGCCGCCATGGCACCAGAAACCTTTCCCGACGACATCGATTTCGGATCACCGGTCGGCGAGGAAATCTGG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NB02

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NA96

References