Detailed information of TA system TA064658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1348921..1349492
• Replicon: chromosome
• Accession: NZ_CP016456
• Organism: Sphingobium sp. RAC03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BSY17_RS20940
• Protein ID: WP_083217110.1
• Coordinates: 1349115..1349492 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A013XX06
• Locus tag: BSY17_RS20935
• Protein ID: WP_043150025.1
• Coordinates: 1348921..1349118 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSY17_RS11080	1344321..1345808	+	1488	WP_069065546.1	DNA recombination protein RmuC	-
BSY17_RS11085	1346131..1346760	+	630	WP_069065547.1	nicotinate-nucleotide adenylyltransferase	-
BSY17_RS11090	1346773..1347162	+	390	WP_037475965.1	ribosome silencing factor	-
BSY17_RS11095	1347204..1347626	+	423	WP_069065548.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
BSY17_RS11100	1347631..1348866	+	1236	WP_069065549.1	peptidoglycan DD-metalloendopeptidase family protein	-
BSY17_RS20935	1348921..1349118	+	198	WP_043150025.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BSY17_RS20940	1349115..1349492	+	378	WP_083217110.1	type II toxin-antitoxin system VapC family toxin	Toxin
BSY17_RS11105	1349596..1350948	+	1353	WP_069066913.1	S41 family peptidase	-
BSY17_RS11110	1351024..1351509	+	486	WP_069065550.1	disulfide bond formation protein B	-
BSY17_RS11115	1351506..1352060	+	555	WP_069065551.1	demethoxyubiquinone hydroxylase family protein	-
BSY17_RS11120	1352074..1352613	+	540	WP_069065552.1	hypothetical protein	-
BSY17_RS11125	1352687..1353106	+	420	WP_069065553.1	hypothetical protein	-
BSY17_RS11130	1353346..1353924	+	579	WP_069065554.1	TMEM165/GDT1 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13953.18 Da
• Isoelectric Point: 7.6167

>T64658 WP_083217110.1 NZ_CP016456:1349115-1349492 [Sphingobium sp. RAC03]
MILVDSSVWMDHLRISNPWLAAGLNAGRILGHPFVIGEIALGSLTNRQGVLRMLHKLPEAELAQHREVDALIEQVPLFNR
GIGYVDTHILASVRLTAGSFLWTRDRRLHSIASEMGLAWTPPHPH

Nucleotide

• Length: 378 bp

>T64658 NZ_CP016456:1349115-1349492 [Sphingobium sp. RAC03]
ATGATATTGGTCGATAGTTCGGTCTGGATGGATCATCTACGCATATCAAACCCATGGCTGGCGGCTGGTTTGAACGCTGG
AAGAATATTGGGCCATCCGTTTGTCATCGGCGAGATCGCGCTGGGCAGTCTGACAAACAGGCAGGGTGTGTTGCGTATGT
TGCACAAATTGCCCGAAGCCGAGCTGGCACAACATCGCGAAGTCGATGCCTTGATCGAACAAGTGCCGCTGTTCAACCGA
GGTATAGGGTATGTCGATACGCACATTCTGGCGTCTGTGCGGCTTACCGCTGGATCATTCCTATGGACACGCGACCGACG
ACTGCACAGTATCGCTTCGGAAATGGGCCTGGCCTGGACGCCACCTCATCCCCATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7168.29 Da
• Isoelectric Point: 6.3084

>AT64658 WP_043150025.1 NZ_CP016456:1348921-1349118 [Sphingobium sp. RAC03]
MRTTLALDDDLVAEAQELTGLKEKSALVREALKALVQREAARRLALLGGSEPDFELPPRRRSVPV

Nucleotide

• Length: 198 bp

>AT64658 NZ_CP016456:1348921-1349118 [Sphingobium sp. RAC03]
ATGCGCACGACATTGGCTCTTGATGACGATCTGGTTGCTGAAGCACAGGAACTGACCGGCTTGAAGGAAAAATCTGCCCT
CGTTCGCGAAGCGCTCAAAGCCCTAGTGCAGCGGGAAGCTGCACGTCGCCTGGCATTACTCGGCGGCTCGGAACCCGATT
TCGAGTTGCCGCCGCGGAGGCGATCAGTTCCGGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A013XX06