Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 34640..35174 Replicon plasmid pBSY17_4
Accession NZ_CP016454
Organism Sphingobium sp. RAC03

Toxin (Protein)


Gene name relE Uniprot ID W1S6F4
Locus tag BSY17_RS04185 Protein ID WP_004212931.1
Coordinates 34640..34921 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J8A6F9
Locus tag BSY17_RS04190 Protein ID WP_011950390.1
Coordinates 34908..35174 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY17_RS04150 29955..30515 + 561 WP_011950653.1 recombinase family protein -
BSY17_RS04155 30510..31577 - 1068 WP_011950654.1 TolC family protein -
BSY17_RS04160 31635..31967 - 333 WP_011950386.1 hypothetical protein -
BSY17_RS04165 32259..32621 + 363 WP_041380198.1 hypothetical protein -
BSY17_RS04170 32659..33054 + 396 WP_041380118.1 CopG family transcriptional regulator -
BSY17_RS04175 33051..34013 + 963 WP_041380120.1 tyrosine-type recombinase/integrase -
BSY17_RS04180 34010..34630 - 621 WP_019086510.1 histidine phosphatase family protein -
BSY17_RS04185 34640..34921 - 282 WP_004212931.1 type II toxin-antitoxin system YafQ family toxin Toxin
BSY17_RS04190 34908..35174 - 267 WP_011950390.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BSY17_RS04195 35593..36222 + 630 WP_011950391.1 transcription elongation protein SprT -
BSY17_RS04200 36305..36958 + 654 WP_011950392.1 AAA family ATPase -
BSY17_RS04205 36955..37269 + 315 WP_011950393.1 hypothetical protein -
BSY17_RS04215 37833..38699 - 867 WP_006953933.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..49737 49737


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(2-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-84)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10634.98 Da        Isoelectric Point: 8.0689

>T64654 WP_004212931.1 NZ_CP016454:c34921-34640 [Sphingobium sp. RAC03]
VRTIDRSTRFKRDYKRESKGRHRATLDADLLPVLAALATDTPLDPKYRDHDLSGDWAGYRDCHVKPDLVLIYAKPDDETL
RLARLGSHSEVFG

Download         Length: 282 bp

>T64654 NZ_CP016454:c34921-34640 [Sphingobium sp. RAC03]
GTGCGGACGATTGATCGTTCGACCCGGTTCAAGCGCGACTACAAGCGGGAATCAAAGGGCCGGCATCGAGCGACCCTCGA
TGCCGATCTTCTGCCTGTCCTGGCCGCGCTCGCGACCGACACGCCGCTTGATCCCAAATACCGCGACCATGATCTGAGCG
GCGATTGGGCGGGCTACCGCGATTGCCACGTCAAGCCCGATCTGGTGCTGATCTACGCCAAGCCCGACGACGAAACGCTG
CGCCTCGCGCGCCTTGGCTCGCATAGCGAGGTATTCGGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9467.92 Da        Isoelectric Point: 6.3194

>AT64654 WP_011950390.1 NZ_CP016454:c35174-34908 [Sphingobium sp. RAC03]
MAVSDTYVRARIDNTTKERATAALGAMGLSISDAIRLLMLRIADERRLPFEVKVPNAATREAMAELAAGKGTKFASVDAL
MADLRADD

Download         Length: 267 bp

>AT64654 NZ_CP016454:c35174-34908 [Sphingobium sp. RAC03]
ATGGCTGTTTCCGACACCTATGTCCGCGCCAGGATCGACAATACGACGAAGGAACGCGCCACCGCCGCGCTGGGCGCGAT
GGGCCTTTCGATCTCCGACGCTATCCGACTGTTGATGCTGCGTATCGCCGACGAACGGCGCTTGCCTTTCGAGGTCAAGG
TTCCGAACGCCGCGACACGCGAGGCAATGGCAGAGCTGGCGGCGGGGAAGGGGACCAAGTTCGCCAGCGTCGATGCGCTG
ATGGCGGACCTCCGTGCGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W1S6F4

Antitoxin

Source ID Structure
AlphaFold DB A0A0J8A6F9

References